Features of Host Cell Invasion by Different Infective Forms of Trypanosoma cruzi
Suppl. I: 135-137
Renato A Mortara+, Daniela O Procópio, Helena C Barros, Newton V Verbisck, Walter K Andreoli, Ricardo BS Silva, Solange da Silva
Disciplina de Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu 862, 6º andar, 04023-062 São Paulo SP Brasil
Through its life cycle from the insect vector to mammalian hosts Trypanosoma cruzi has developed clever strategies to reach the intracellular milieu where it grows sheltered from the hosts' immune system. We have been interested in several aspects of in vitro interactions of different infective forms of the parasite with cultured mammalian cells. We have observed that not only the classically infective trypomastigotes but also amastigotes, originated from the extracellular differentiation of trypomastigotes, can infect cultured cells. Interestingly, the process of invasion of different parasite infective forms is remarkably distinct and also highly dependent on the host cell type.
Key words: Trypanosoma cruzi - cell invasion - amastigote - trypomastigote
In the natural course of infection, the infected insect transmits metacyclic trypomastigotes that invade mammalian cells, escape the endocytic vacuole and transform into rounded amastigote forms. Amastigotes replicate in the cytoplasm, differentiate back into trypomastigotes that are released after cell lysis. An alternative sub-cycle can occur in the mammalian host if amastigotes, originated from premature lysis of infected cells (Behbehani 1973, Nogueira & Cohn 1976, Carvalho et al. 1981, Umezawa et al. 1985, Ulisses de Carvalho & De Souza 1986) or by extracellular differentiation of trypomastigotes (Pan 1978, Andrews et al. 1987, Ley et al. 1988, Mortara 1991), invade professional or non-professional phagocytes. Inside these cells the amastigotes can survive and sustain the parasite's life cycle. These amastigote-like infective forms present morphological, immunological, biochemical and ultrastructural similarities to intracellular amastigotes (Villalta & Kierszenbaum, 1982, 1984, Hudson et al. 1984, Andrews et al. 1987, Ley et al. 1988) and express specific molecular markers of amastigotes (Andrews et al. 1987, Lima et al. 1988, Pan & McMahon-Pratt 1989). We have seen that extracellular amastigotes are able to infect HeLa cells by interacting with microvilli on the dorsal surface of these cells (Mortara 1991). This association leads to microvillus aggregation that can be followed by microfilament clustering observed by phalloidin staining. By contrast, trypomastigotes preferentially enter HeLa cells at the edges showing that the different forms of the parasite interact with specific regions of the cell surface (Mortara 1991). Differences in invasion mechanisms utilized by extracellular amastigotes and metacyclic trypomastigotes became even more apparent when we tested the requirement for integrity of host cells' cytoskeleton. For that HeLa and Vero cells were treated with drugs that disrupt microfilaments or microtubules. Mammalian cells with altered expression in actin-binding proteins (Actin-Binding Protein, ABP, and gelsolin) were also tested as target cells. Protein kinase inhibitors (staurosporine and genistein) interfered with the invasion processes. The distribution of different host cell components during the invasion by the two parasite forms was consistent with the emerging notion that extracellular amastigotes and metacyclic trypomastigotes utilize mechanisms to invade host cells with highly distinctive features for each Trypanosoma cruzi form and for each host cell. Preliminary data from our studies also suggest that culture-derived trypomastigotes and metacyclic trypomastigotes may form parasito-phorous vacuoles with distinct properties.
MATERIALS AND METHODS
Vero, HeLa, Actin-binding mutants and transfectants, gelsolin transfectants, Coxiella burnetti-chronically infected Vero cells were routinely grown in DME or RPMI-1640 media supplemented with fetal calf serum and antibiotics when necessary. Parasites from different T. cruzi strains were maintained in LIT medium and trypo-mastigotes generated by infecting Vero cells. Amastigotes were obtained from infected cells (intracellular) or after 48 hr incubation of tissue-culture trypomastigotes in LIT medium at 37ºC (extracellular).
+Corresponding author. Fax: +55-11-571-1095. E-mail: renato.dmip@epm.br
Received 9 June 1999
Accepted 9 August 1999
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