Mem Inst Oswaldo Cruz, Rio de Janeiro, 112(7) July 2017
Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions
1Universidade Federal do Rio Grande, Escola de Medicina, Laboratório de Biologia Molecular, Rio Grande, RS, Brasil
2Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil
3Universidade Federal do Rio Grande, Escola de Medicina, Centro de Obstetrícia e Ginecologia, Rio Grande, RS, Brasil
4Policlínica de Assistência Médica, Rio Grande, RS, Brasil
BACKGROUND Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer.
OBJECTIVES This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions.
METHODS HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were sequenced, and characteristic single nucleotide variants were identified.
FINDINGS A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%) and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with 88% defined as lineage A, 10% as lineage D, and 2% as lineage B.
MAIN CONCLUSIONS This study identified a high frequency of European and North American HPV16 lineages, consistent with the genetic background of the human population in southern Brazil.