Mem Inst Oswaldo Cruz, Rio de Janeiro, 112(10) October 2017
Genome announcement and highlight

Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil

Gisele R de Oliveira1,2, Juliana D Siqueira2, Fabiana Finger-Jardim1, Valdimara C Vieira2, Ronald L Silva3, Carla V Gonçalves4, Esmeralda A Soares2, Ana Maria Barral de Martinez1, Marcelo A Soares2,+

1Universidade Federal do Rio Grande, Escola de Medicina, Laboratório de Biologia Molecular, Rio Grande, RS, Brasil
2Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil
3Universidade Federal do Rio Grande, Escola de Medicina, Rio Grande, RS, Brasil
4Universidade Federal do Rio Grande, Escola de Medicina, Centro de Obstetrícia e Ginecologia, Rio Grande, RS, Brasil

DOI: 10.1590/0074-02760170121
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ABSTRACT

The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences in association with this disease. In this work, we have characterised complete HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 complete genomes from 13 different HPV types were assembled. Phylogenetic and genetic distance analyses were performed to classify the HPV genomes into lineages and sublineages. This is the first report describing the distribution of HPV intratype lineages of high and low oncogenic risk in asymptomatic women from southern Brazil.

Financial support: FAPERJ (grant # E25/203.571/2014), CNPq (grant # 460346/2014-8), FAPERGS (grant # 33662551/11-3).
+ Corresponding author: masoares@inca.gov.br
Received 28 March 2017
Accepted 8 May 2017

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