Mem Inst Oswaldo Cruz, Rio de Janeiro, 113(5) May 2018
Genome announcements and highlights

Genomic characterisation of Leptospira inadai serogroup Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

Luisa Z Moreno1, Fabiana Miraglia2, Ana P Loureiro2, Frederico S Kremer3, Luisa Z Moreno1, Fabiana Miraglia2, Ana P Loureiro2, Frederico S Kremer3, Marcus R Eslabao3, Odir A Dellagostin3, Walter Lilenbaum2, Silvio A Vasconcellos1, Marcos B Heinemann1, Andrea M Moreno1,+

1Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Epidemiologia Molecular e Resistência a Antimicrobianos, São Paulo, SP, Brasil
2Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia, Laboratório de Bacteriologia Veterinária, Niterói, RJ, Brasil
3Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Pelotas, RS, Brasil

DOI: 10.1590/0074-02760170444
28 views 37 downloads

Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ 10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.

01. Alikhan NF, Petty NK, Ben Zakour NL, Beatson SA. BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons. BMC Genomics. 2011; 12: 402.
02. Arzouni JP, Parola P, La Scola B, Postic D, Brouqui P, Raoult D. Human infection caused by Leptospira fainei. Emerg Infect Dis. 2002; 8(8): 865-8.
03. Bourhy P, Collet L, Brisse S, Picardeau M. Leptospira mayottensis sp. nov., a pathogenic species of the genus Leptospira isolated from humans. Int J Syst Evol Microbiol. 2014; 64(12): 4061-7.
04. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, Parkhill J. ACT: the Artemis Comparison Tool. Bioinformatics. 2005; 21(16): 3422-3.
05. Chiriboga J, Barragan V, Arroyo G, Sosa A, Birdsell DN, España K, et al. High prevalence of intermediate Leptospira spp. DNA in febrile humans from urban and rural Ecuador. Emerg Infect Dis. 2015; 21(12): 2141-7.
06. Darling AE, Mau B, Perna NT. progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS ONE. 2010; 5(6): e11147.
07. Evangelista KV, Coburn J. Leptospira as an emerging pathogen: a review of this biology, pathogenesis and host immune responses. Fut Microbiol. 2010; 5(9): 1413-25.
08. Fouts DE, Matthias MA, Adhikarla H, Adler B, Amorim-Santos L, Berg DE, et al. What makes a bacterial species pathogenic? Comparative genomic analysis of the genus Leptospira. PLoS Negl Trop Dis. 2016; 10(2): e0004403.
09. Gangadhar NL, Prabhudas K, Bhushan S, Sulthana M, Barbuddhe SB, Rehaman H. Leptospira infection in animals and humans: a potential public health risk in India. Rev Sci Tech. 2008; 27(3): 885-92.
10. Gangadhar NL, Rajasekhar M, Smythe LD, Norris MA, Symonds ML, Dohnt MF. Reservoir hosts of Leptospira inadai in India. Rev Sci Tech. 2000; 19(3): 793-9.
11. Levett P, Morey R, Galloway R, Steigerwalt A. Leptospira broomii sp. nov., isolated from humans with leptospirosis. Int J Syst Evol Microbiol. 2006; 56(Pt 3): 671-3.
12. Levett PN. Leptospirosis. Clin Microbiol Rev. 2001; 14(2): 296-326.
13. Matthias MA, Ricaldi JN, Cespedes M, Diaz MM, Galloway RL, Saito M, et al. Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. PLoS Negl Trop Dis. 2008; 2(4): e213.
14. Richter M, Rosselló-Móra R, Glöckner FO, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics. 2016; 32(6): 929-31.
15. Schmid GP, Steere AC, Kornblatt AN, Kaufmann AF, Moss CW, Johnson RC, et al. Newly recognized Leptospira species (‘‘Leptospira inadai’’ serovar lyme) isolated from human skin. J Clin Microbiol. 1986; 24(3): 484-6.
16. Slack AT, Kalambaheti T, Symonds ML, Dohnt MF, Galloway RL, Steigerwalt AG, et al. Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand. Int J Syst Evol Microbiol. 2008; 58(Pt 10): 2305-8.
17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony method. Mol Biol Evol. 2011; 28(10): 2731-9.
18. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res. 2016; 44(14): 6614-24.
19. Yasuda PH, Steigerwalt AG, Sulzer KR, Kaufmann AF, Rogers F, Brenner DJ. Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int J Syst Bacteriol. 1987; 37(4): 407-15.
20. Zakeri S, Khorami N, Ganji ZF, Sepahian N, Malmasi AA, Gouya MM, et al. Leptospira wolffii, a potential new pathogenic Leptospira species detected in human, sheep and dog. Infect Genet Evol. 2010; 10(2): 273-7.

Financial support: CNPq, CAPES, FAPESP (2011/18290-0, 2013/17136-2).
+ Corresponding author:
Received 17 October 2017
Accepted 6 February 2018

Our Location

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco 
sala 201, Manguinhos, 21040-900 
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This email address is being protected from spambots. You need JavaScript enabled to view it.

Support Program

logo fiocruz logo governo
logo faperj logo cnpq marca capes