Mem Inst Oswaldo Cruz, Rio de Janeiro, 113(5) May 2018
Full paper

Evaluation of the recombinant antigens Wb14 and WbTEvaluation of the recombinant antigens Wb14 and WbTfor the capture antibody diagnosis of lymphatic filariasis

André Filipe Pastor1,2,4,+, Abraham Rocha1,3,5, Klécia de Melo Cassemiro6, Marli Tenório1,André Filipe Pastor1,2,4,+, Abraham Rocha1,3,5, Klécia de Melo Cassemiro6, Marli Tenório1, Paula Melo1,3, Maria Rosângela Grilis1,3, Maressa Rhuama1,2, Antonio Mauro Rezende1,Osvaldo Pompilio de Melo Neto1,2, Ernesto Marques Jr1, Rafael Dhalia1,2

1Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Recife, PE, Brasil
2Universidade Federal de Pernambuco, Recife, PE, Brasil
3Fundação Oswaldo Cruz-Fiocruz, Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses, Recife, PE, Brasil
4Instituto Federal de Ciência e Tecnologia do Sertão Pernambucano, Campus Floresta, PE, Brasil
5Hospital Otávio de Freitas, Recife, PE, Brasil6Instituto Nacional de Câncer, Labratório de Patologia Clínica, Rio de Janeiro, RJ, Brasil

DOI: 10.1590/0074-02760170435
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BACKGROUND Lymphatic filariasis (LF) is a parasitic disease caused mainly by the Wuchereria bancrofti worm and that affects up to 120 million people worldwide. LF is the second cause of chronic global deformity, responsible for 15 million people with lymphedema (elephantiasis) and 25 million men with scrotal hydrocele. Its diagnosis is still associated with numerous difficulties, such as the sample collection periods (microfilaria nocturnal periodicity) and limited diagnostic kits.

OBJECTIVES The aim of this work was to evaluate two recombinant antigens (Wb14 and WbT) as part of an enzyme-linked immunosorbent assay (ELISA) based antibody capture tests for LF.

METHODS The recombinant antigens rWb14 and rWbT were expressed in Escherichia coli BL21 and an antibody capture ELISA was performed. For this, sera were used from microfilaremic individuals with W. bancrofti (MF), chronic pathology (CP), individuals infected with Strongyloides (SP) and healthy controls from endemic (EN) and non-endemic (NE) areas.

FINDINGS Both tests showed similar results, with 90% sensitivity and 96.6% specificity. In comparison with the BM14 ELISA commercial test, the Wb14 and WbT antigens performed with identical sensitivity but greater specificity. Reduced positivity with the CP suggested a potential to monitor cure. This was not confirmed, however, when sera from individuals up to seven years after treatment were assayed.

MAIN CONCLUSIONS The Wb14 and WbT ELISAs were considered efficient and promising diagnostic tests. Due to the importance of antibody capture analysis to evaluate the Global Program to Eliminate Lymphatic Filariasis (GPELF), the tests proposed here appear as great alternatives to the available commercial system.

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Financial support: FACEPE (APQ-0058-4.00/13), FIOCRUZ-PE.Financial support: FACEPE (APQ-0058-4.00/13), FIOCRUZ-PE.
+ Corresponding author:
Received 12 October 2017
Accepted 16 February 2018

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