MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(10) October 2017
PAGES: 698-708 DOI: 10.1590/0074-02760160519 Full paper
The eco-epidemiology of Triatoma infestans in the temperate Monte Desert ecoregion of mid-western Argentina

Ana Laura Carbajal-de-la-Fuente1,+, Yael Mariana Provecho1, María del Pilar Fernández1, Marta Victoria Cardinal1, Patricia Lencina2,3, Cynthia Spillmann4, Ricardo Esteban Gürtler1

1University of Buenos Aires, Institute of Ecology, Genetics and Evolution of Buenos Aires, Faculty of Exact and Natural Sciences, National Scientific and Technical Research Council, Ciudad Autónoma de Buenos Aires, Argentina
2Health Ministry, Division of Zoonoses, Vectors and Reservoirs, Mendoza, Argentina
3Health Ministry, Laboratory of Public Health, Mendoza, Argentina
4National Health Ministry, National Chagas Program, Córdoba, Argentina

Abstract

BACKGROUND The eco-epidemiological status of Chagas disease in the Monte Desert ecoregion of western Argentina is largely unknown. We investigated the environmental and socio-demographic determinants of house infestation with Triatoma infestans, bug abundance, vector infection with Trypanosoma cruzi and host-feeding sources in a well-defined rural area of Lavalle Department in the Mendoza province.

METHODS Technical personnel inspected 198 houses for evidence of infestation with T. infestans, and the 76 houses included in the current study were re-inspected. In parallel with the vector survey, an environmental and socio-demographic survey was also conducted. Univariate risk factor analysis for domiciliary infestation was carried out using Firth penalised logistic regression. We fitted generalised linear models for house infestation and bug abundance. Blood meals were tested with a direct ELISA assay, and T. cruzi infection was determined using a hot-start polymerase chain reaction (PCR) targeting the kinetoplast minicircle (kDNA-PCR).

FINDINGS The households studied included an aged population living in precarious houses whose main economic activities included goat husbandry. T. infestans was found in 21.2% of 198 houses and in 55.3% of the 76 re-inspected houses. Peridomestic habitats exhibited higher infestation rates and bug abundances than did domiciles, and goat corrals showed high levels of infestation. The main host-feeding sources were goats. Vector infection was present in 10.2% of domiciles and 3.2% of peridomiciles. Generalised linear models showed that peridomestic infestation was positively and significantly associated with the presence of mud walls and the abundance of chickens and goats, and bug abundance increased with the number of all hosts except rabbits.

MAIN CONCLUSIONS We highlight the relative importance of specific peridomestic structures (i.e., goat corrals and chicken coops) associated with construction materials and host abundance as sources of persistent bug infestation driving domestic colonisation. Environmental management strategies framed in a community-based programme combined with improved insecticide spraying and sustained vector surveillance are needed to effectively suppress local T. infestans populations.

Financial support: National Chagas Control Program, National Health Ministry; the Division of Zoonoses, Vectors and Reservoirs, Health Ministry, Mendoza; the Technologic and Social Development Projects (PDTS-PX03), University of Buenos Aires; and Argentina’s National Promotion Agency for Science and Technology (PICTs 2013-2538 and 2014-3746).
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 2 December 2016
Accepted 22 April 2017

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