MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 112 | 2017
PAGES: DOI: 10.1590/0074-02760170083 Full paper
Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras

Osbourne Quaye1,2,+, Sunando Roy1, Kunchala Rungsrisuriyachai1, Mathew D Esona1, Ziqian Xu3, Ka Ian Tam1, Dina J Castro Banegas4, Gloria Rey-Benito5, Michael D Bowen1

1Centers for Disease Control and Prevention, Gastroenteritis and Respiratory Viruses Laboratory Branch, Atlanta, Georgia, USA
2University of Ghana, Department of Biochemistry, Cell and Molecular Biology, West African Center for Cell Biology of Infectious Pathogens, Legon, Accra, Ghana
3China Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, China
4Nacional Colonia La Campaña, Tegucigalpa, Honduras
5Pan American Health Organization, Washington DC, USA

Abstract

BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains.

OBJECTIVES In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain.

METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0.

FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain.

Financial support: PAHO, WHO, CDC.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 28 February 2017
Accepted 24 August 2017

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