MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(3) March 2017
PAGES: 209-213 DOI: 10.1590/0074-02760160394 Short communication
Poor sensitivity of rapid tests for the detection of antibodies to the hepatitis B virus: implications for field studies

Helena Medina Cruz1, Leticia de Paula Scalioni1, Vanessa Salete de Paula2, Juliana Custódio Miguel1, Kycia Maria Rodrigues do Ó3, Flavio Augusto Pádua Milagres4, Marcelo Santos Cruz5, Francisco Inácio Bastos6, Priscila Pollo Flores7, Erotildes Leal8, Ana Rita Coimbra Motta-Castro9, Lia Laura Lewis-Ximenez1, Elisabeth Lampe1, Livia Melo Villar1,+

1Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Hepatites Virais, Rio de Janeiro, RJ, Brasil
2Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Desenvolvimento Tecnológico em Virologia, Rio de Janeiro, RJ, Brasil
3Hospital São Lucas, Petrópolis, RJ, Brasil
4Universidade Federal do Tocantins, Faculdade de Medicina, Palmas, TO, Brasil
5Universidade Federal do Rio de Janeiro, Instituto de Psiquiatria, Rio de Janeiro, RJ, Brasil
6Fundação Oswaldo Cruz-Fiocruz, Instituto de Comunicação e Informação Científica e Tecnológica em Saúde, Rio de Janeiro, RJ, Brasil
7Universidade de Brasília, Brasília, DF, Brasil
8Universidade Federal do Rio de Janeiro, Campus Macaé, Macaé, RJ, Brasil
9Universidade Federal do Mato Grosso do Sul, Departamento de Bioquímica e Farmácia, Campo Grande, MS, Brasil

Abstract

Rapid tests (RTs) can be used as an alternative method for the conventional diagnosis of hepatitis B virus (HBV). This study aims to evaluate antibodies to HBsAg (anti-HBs) and antibodies to HBeAg (anti-HBe) RTs under different Brazilian settings. The following three groups were included: GI: viral hepatitis outpatient services; GII: low resource areas; and GIII: crack users and beauticians. Imuno-rápido anti-HBsAg™ and Imuno-rápido anti-HBeAg™ RTs were evaluated and showed specificities greater than 95% in all groups. The sensitivity values to anti-HBs were 50.38%, 51.05% and 46.73% and the sensitivity values to anti-HBe were 76.99%, 10.34% and 11.76% in the GI, GII and GIII groups, respectively. The assays had a low sensitivity and high specificity, which indicated their use for screening in regions endemic for HBV.

Financial support: FAPERJ, CNPq, CAPES, FIOCRUZ.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 28 August 2016
Accepted 24 November 2016

CONTACT US

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco
sala 201, Manguinhos, 21040-900
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

twitterfacebook

SUPPORT PROGRAM

marca fiocruzmarca brasil
marca faperjmarca cnpqmarca capes n