Eloise Brasil Moraes1,2,+, Letícia Slompo1, Amanda Juliane Finardi1,2, Heloisa Paro Pedro da Silveira3, Luciana Ruiz4, Harrison Magdinier Gomes5, Virginia Bodelão Richini4, Philip Suffys5, Carlos Magno Castelo Branco Fortaleza2, Ricardo Cavalcanti2, Ida Maria Foschiani Dias Baptista1,2
1Instituto Lauro de Souza Lima, Divisão de Pesquisa e Ensino, Equipe Técnica de Microbiologia, Bauru, SP, Brasil
2Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, Doenças Tropicais, Botucatu, SP, Brasil
3Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, Núcleo de Ciências Biomédicas, São José do Rio Preto, SP, Brasil
4Instituto Adolfo Lutz, Centro de Laboratório Regional de Bauru, Núcleo de Ciências Biomédicas, Bauru, SP, Brasil
5Fundação Oswaldo Cruz-Fiocruz, Laboratório de Biologia Molecular aplicada a Micobactérias, Rio de Janeiro, RJ, Brasil
BACKGROUND Tuberculosis (TB) continues to be a disease that affects many countries around the world, including Brazil. Recently, a subtype of Latin American-Mediterranean family strain was identified and characterised by RDRio. The strain has been associated with different characteristics of the disease.
OBJECTIVES In the present study we investigated the association of epidemiological, clinical, radiological and bacteriological variables with pulmonary tuberculosis caused by RDRio Mycobacterium tuberculosis strain in large regions of São Paulo.
METHODS We conducted a cross-sectional study in 530 patients with pulmonary tuberculosis, diagnosed using sputum culture, from two regions of the São Paulo state in Brazil. The samples were brought to São Paulo reference laboratories for epidemiological, clinical, radiological and bacteriological analyses, and the data were obtained from a TB notification system. RDRio genotyping and Spoligotyping of the samples were performed. For the analysis of the categorical variables we used the chi-square test or the Fisher’s exact test, and for the continuous variables, the Mann-Whitney test. In addition, a logistic regression was used for multivariate analysis. Differences with p < 0.05 were considered significant.
FINDINGS The RDRio deletion was identified in 152 (28.7%) samples. In the univariate analysis, both the age groups above 25 years and alcohol consumption were associated with the RDRio deletion. The multivariate analysis confirmed the association of the RDRio deletion with the age groups: 25-35 years old [OR: 2.28 (1.02-5.07; p = 0.04)] and 36-60 years old (OR: 2.36 (1.11-5.05); p = 0.03], and also with alcohol consumption [OR: 1.63 (1.05-2.54); p = 0,03].
MAIN CONCLUSIONS In this study, we identified new factors associated with the M. tuberculosis of the RDRio deletion strains infection.
Financial support: FAPESP (nº 2013/09538-3)
Received 30 July 2016
Accepted 7 November 2016