MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(3) March 2017
PAGES: 220-223 DOI: 10.1590/0074-02760160354 Technical notes
Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction

Lyana Rodrigues Pinto Lima1, Amanda Perse da Silva1, Jonas Schmidt-Chanasit2, Vanessa Salete de Paula1,+

1Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Desenvolvimento Tecnológico em Virologia, Rio de Janeiro, RJ, Brasil
2Bernhard Nocht Institute for Tropical Medicine, Diagnostic Virology Laboratory, Hamburg, Germany

Abstract

The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.

Financial support: PAPES VI (407430/2012-1 - APQ), FAPERJ.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 4 August 2016
Accepted 23 November 2016

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