MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(3) March 2017
PAGES: 214-219 DOI: 10.1590/0074-02760160412 Short communication
Comparison of four molecular approaches to identify Candida parapsilosis complex species

Leonardo Silva Barbedo+, Maria Helena Galdino Figueiredo-Carvalho, Mauro de Medeiros Muniz, Rosely Maria Zancopé-Oliveira

Fundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Laboratório de Micologia, Rio de Janeiro, RJ, Brasil

Abstract

Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis.

Financial support: CNPq (350338/2000-0), FAPERJ (E-26/103.157/2011) to RMZ-O, CAPES, PDSE (BEX 19194/12-9), PNPD to LSB.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 15 September 2016
Accepted 8 November 2016

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