MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 112(8) August 2017
PAGES: 537-543 DOI: 10.1590/0074-02760170023 Full paper
Biosynthesized silver nanoparticles: are they effective antimicrobials?

Mudara K Peiris1, Chinthika P Gunasekara1, Pradeep M Jayaweera2, Nuwan DH Arachchi2, Neluka Fernando1/+

1University of Sri Jayewardenepura, Faculty of Medical Sciences, Department of Microbiology, Gangodawila, Nugegoda, Sri Lanka
2University of Sri Jayewardenepura, Faculty of Applied Sciences, Department of Chemistry, Gangodawila, Nugegoda, Sri Lanka

Abstract

BACKGROUND Silver nanoparticles (AgNPs) are increasingly being used in medical applications. Therefore, cost effective and green methods for generating AgNPs are required.

OBJECTIVES This study aimed towards the biosynthesis, characterisation, and determination of antimicrobial activity of AgNPs produced using Pseudomonas aeruginosa ATCC 27853.

METHODS Culture conditions (AgNO3 concentration, pH, and incubation temperature and time) were optimized to achieve maximum AgNP production. The characterisation of AgNPs and their stability were evaluated by UV-visible spectrophotometry and scanning electron microscopy.

FINDINGS The characteristic UV-visible absorbance peak was observed in the 420–430 nm range. Most of the particles were spherical in shape within a size range of 33–300 nm. The biosynthesized AgNPs exhibited higher stability than that exhibited by chemically synthesized AgNPs in the presence of electrolytes. The biosynthesized AgNPs exhibited antimicrobial activity against Escherichia coli, P. aeruginosa, Salmonella typhimurium, Staphylococcus aureus, methicillin-resistant S. aureus, Acinetobacter baumannii, and Candida albicans.

MAIN CONCLUSION As compared to the tested Gram-negative bacteria, Gram-positive bacteria required higher contact time to achieve 100% reduction of colony forming units when treated with biosynthesized AgNPs produced using P. aeruginosa.

Financial support: University of Sri Jayewardenepura (ASP/01/RE/MED/2016/42)
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 20 January 2017
Accepted 23 March 2017

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