MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 112 | 2017
PAGES: DOI: 10.1590/0074-02760160494 Full paper
Antifungal activity of caspofungin in experimental infective endocarditis caused by Candida albicans

Gerardo Becerra Victorio1, Lorena Michele Brennan Bourdon2, Leonel García Benavides3, Selene G Huerta-Olvera4, Arturo Plascencia5, José Villanueva6, Erika Martinez-Lopez6, Iván Isidro Hernández-Cañaveral7,+

1Universidad Autónoma de Chiapas, Facultad de Ciencias Químicas, Chiapas, Mexico
2Comisión para la Protección contra Riesgos Sanitarios del Estado de Jalisco, Guadalajara, Mexico
3Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Departamento de Fisiología, Guadalajara, Jalisco, Mexico
4Universidad de Guadalajara, Centro Universitario de la Ciénega, Departamento de Ciencias Médicas y de la Vida, Ocotlan, Jalisco, Mexico
5Hospital Civil Fray Antonio Alcalde, Servicio de Infectología Pediátrica, Guadalajara, Jalisco, Mexico
6Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Departamento de Biología Molecular y Genómica, Guadalajara, Jalisco, Mexico
7Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Departamento de Microbiología y Patología, Guadalajara, Jalisco, Mexico

Abstract

BACKGROUND Infective endocarditis is a disease characterised by heart valve lesions, which exhibit extracellular matrix proteins that act as a physical barrier to prevent the passage of antimicrobial agents. The genus Candida has acquired clinical importance given that it is increasingly being isolated from cases of nosocomial infections.

OBJECTIVE To evaluate the activity of caspofungin compared to that of liposomal amphotericin B against Candida albicans in experimental infective endocarditis.

METHODS Wistar rats underwent surgical intervention and infection with strains of C. albicans to develop infective endocarditis. Three groups were formed: the first group was treated with caspofungin, the second with liposomal amphotericin B, and the third received a placebo. In vitro sensitivity was first determined to further evaluate the effect of these treatments on a rat experimental model of endocarditis by semiquantitative culture of fibrinous vegetations and histological analysis.

FINDINGS Our semiquantitative culture of growing vegetation showed massive C. albicans colonisation in rats without treatment, whereas rats treated with caspofungin showed significantly reduced colonisation, which was similar to the results obtained with liposomal amphotericin B.

CONCLUSIONS The antifungal activity of caspofungin is similar to that of liposomal amphotericin B in an experimental model of infective endocarditis caused by C. albicans.

+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 11 November 2016
Accepted 9 January 2017

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