MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 112 | 2017
PAGES: DOI: 10.1590/0074-02760160438 Full paper
Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis

Lara Saraiva1,+, Camila Gonçalves Leite2, Ana Cristina Vianna Mariano da Rocha Lima1, Luiz Otávio Alves de Carvalho2, Agnes Antônia Sampaio Pereira1, Jerônimo Marteleto Nunes Rugani1, Felipe Dutra Rego1, Célia Maria Ferreira Gontijo1, José Dilermando Andrade Filho1

1Fundação Oswaldo Cruz-Fiocruz, Centro de Pesquisas René Rachou, Grupo de Estudos em Leishmanioses, Belo Horizonte, MG, Brasil
2Prefeitura Municipal de Belo Horizonte, Gerência de Controle de Zoonoses, Belo Horizonte, MG, Brasil

Abstract

BACKGROUND Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis.

OBJECTIVES This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population.

METHODS The sampling was carried out using automatic HP light traps at all covered areas of the Venda Nova SD, in a total of eighteen light traps. Sampled specimens were identified following Galati (2003), and females were submitted to molecular techniques for the detection and identification of Leishmania DNA. A simple environmental description was done for it area and Kernel estimation was used to infer vector density for each study site.

FINDINGS A total of 2,427 sand fly specimens belonging to eight species and five genera were collected of which 95.3% were Lutzomyia longipalpis. The seasonal variation curve was delineated by this species. Lu. longipalpis was the most abundant at all collection points and in all months of the study, and exhibited a natural infection rate of 1.01% for Leishmania infantum and 1.77% for Leishmania braziliensis.

MAIN CONCLUSIONS The results show the presence and adaptation of Lu. longipalpis to the anthropic environment of BH and reinforces its role as the main vector of L. infantum in the region.

Financial support: CNPq, FAPEMIG.
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 30 September 2016
Accepted 9 December 2016

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