MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 112 | 2017
PAGES: DOI: 10.1590/0074-02760160341 Short communication
Experimental toxocariasis in BALB/c mice: relationship between parasite inoculum and the IgG immune response

Gabriela Rodrigues e Fonseca1,4, Sergio Vieira dos Santos1,2, Pedro Paulo Chieffi2, Fabiana Martins de Paula1,3, Ronaldo Cesar Borges Gryschek1,3, Susana Angélica Zevallos Lescano1,3,+

1Instituto de Medicina Tropical de São Paulo, São Paulo, SP, Brasil
2Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP, Brasil
3Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Laboratório de Investigação Médica, São Paulo, SP, Brasil
4Universidade de São Paulo, Faculdade de Medicina, Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, São Paulo, SP, Brasil

Abstract

BALB/c mice were inoculated with 5-500 Toxocara canis infective eggs, and bled at 15-120 days post infection (dpi) to evaluate the dynamics of IgG antibody response and larvae distribution. Positive results were observed in all occasions for every inoculum, and a direct proportional relationship between antibody detection and the parasitic load was observed. In samples collected at 60 dpi, detection of IgG was more intense, especially with the 50 and 500 egg doses; also, a correlation between antibody level and egg count was observed with these two inocula. At 120 dpi, a decrease in antibody titer was observed for all groups; and at the end of the experiment, larvae were recovered from carcass, liver and brain. In the liver, larvae were only found in mice inoculated with 500 T. canis eggs. In carcasses, these were recovered in all groups, and the group inoculated with 50 eggs showed the highest percentage of larvae in the brain.

Financial support: FAPESP (grant nº 2014/07345-6).
+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 27 July 2016
Accepted 27 December 2016

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