MEM INST OSWALDO CRUZ, RIO DE JANEIRO, Vol. 112 | 2017
PAGES: DOI: 10.1590/0074-02760160435 Full paper
Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Sandra Yamashiro1, Vagner Ricardo da Silva Fiuza2, Ângela Therezinha Lauand Sampaio Teixeira3, Nilson Branco1, Carlos Emílio Levy3, Isabel Cristina Vidal Siqueira de Castro1, Regina Maura Bueno Franco1,+

1Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Biologia Animal, Laboratório de Protozoologia, Campinas, SP, Brasil
2Universidade Estadual de Campinas, Faculdade de Engenharia Civil, Arquitetura e Urbanismo, Departamento de Saneamento e Ambiente, Campinas, SP, Brasil
3Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Departamento de Patologia Clínica, Hospital de Clínicas, Campinas, SP, Brasil

Abstract

BACKGROUND Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spores, but can also result from direct contact with spores through broken skin, eye lesions, and sexual transmission, depending on the microsporidian species. Although there are reports of E. bieneusi found in humans and animals in Brazil, there are no published studies of environmental samples examined by molecular methods.

OBJECTIVES The purpose of this study was to verify the presence of E. bieneusi in raw sewage and treated effluent from a combined system by molecular methods.

METHODS Raw sewage and treated effluent samples collected from a combined system were analysed for the presence of E. bieneusi using the internal transcriber spacer (ITS) region of E. bieneusi by nested polymerase chain reaction.

FINDINGS The analysis revealed E. bieneusi presence and a novel genotype (EbRB) in one raw sewage sample and one treated effluent.

MAIN CONCLUSIONS The presence of E. bieneusi in final effluent indicates that the combined system may not remove microsporidian spores. This study is the first report of E. bieneusi in environmental samples in Brazil.

+ Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
Received 28 September 2016
Accepted 4 March 2017

CONTACT US

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco
sala 201, Manguinhos, 21040-900
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

twitterfacebook

SUPPORT PROGRAM

marca fiocruzmarca brasil
marca faperjmarca cnpqmarca capes n