MEM INST OSWALDO CRUZ, RIO DE JANEIRO, FAST TRACK
PAGES: DOI: 10.1590/0074-02760170077 Full paper
ZIKA AND CHIKUNGUNYA VIRUS DETECTION IN NATURALLY INFECTED AEDES AEGYPTI IN ECUADOR

Varsovia Cevallos1,+, Patricio Ponce2, Maria José Cárdenas1, Jesse J. Waggoner3, Benjamin A. Pinsky4,5, Josefina Coloma6, Diego Morales1, Cristina Quiroga1

1 Instituto Nacional de Investigación en Salud Pública, Centro Nacional de Referencia e Investigación en Vectores, Quito, Ecuador.
2 Instituto de Biomedicina, Facultad de Biología, Universidad Central del Ecuador, Quito, Ecuador.
3 Emory University, School of Medicine, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.
4 Stanford University School of Medicine, Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford, California, USA.
5 Stanford University, School of Medicine, Department of Pathology, Stanford, California, USA.
6 School of Public Health and Vaccinology, University of California, Berkeley, Berkeley, California, USA.

Abstract

The wide and rapid spread of Chikungunya (CHIKV) and Zika (ZIKV) viruses represent a global public health problem, especially for tropical and subtropical environments. This study was done to detect ZIKV and CHIKV viruses in naturally infected Aedes aegypti mosquitoes from active epidemic urban areas in Ecuador. Pools and individuals of field collected Aedes aegypti mosquitoes from high-risk arboviruses infection sites in Ecuador were analyzed for the presence of CHIKV and ZIKV using RT-PCR. Phylogenetic analysis demonstrated that both ZIKV and CHIKV strains circulating in Ecuador correspond to the Asian lineages. Values of MIR (minimum infection rates) and MLE (maximum likelihood estimation) are high for CHIKV and ZIKV infected mosquitoes in three cities on the Pacific coast of Ecuador. The early detection of CHIKV and ZIKV in mosquitoes may help to understand the dynamics of the diseases in high-risk areas, and to design data based epidemiological surveillance and vector control programs and to activate the preparedness and response of the public health system.

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