MEM INST OSWALDO CRUZ, RIO DE JANEIRO, FAST TRACK
PAGES: DOI: 10.1590/0074-02760170134 Full paper
GENOME ANALYSIS OF YELLOW FEVER VIRUS OF BRAZIL ONGOING OUTBREAK REVEALS POLYMORPHISMS

Myrna C. Bonaldo1,+, Mariela Martínez Gómez1, Alexandre A. C. dos Santos1, Filipe Vieira Santos de Abreu2,3, Anielly Ferreira-de-Brito2, Rafaella Moraes de Miranda2, Marcia Gonçalves de Castro2, Ricardo Lourenço-de-Oliveira2

1Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil
2Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil
3Instituto Federal do Norte de Minas Gerais

Abstract

The current yellow fever (YF) outbreak in Brazil is the most severe recently reported in the country. It has rapidly spread to areas where YF viral activity have not been observed for more than seventy years and vaccine coverage is almost null. Here, we sequenced the whole YF genome of two naturally infected howler-monkeys (Alouatta clamitans) from the Municipality of Domingos Martins, State of Espírito Santo, Brazil. The ongoing-outbreak genome sequences are identical. They clustered in 1E sub-clade (South America I genotype) together with recent Brazilian and Venezuelan strains characterized from infections in humans and non-humans primates. However, we detected eight unique amino acid changes in the viral proteins, which are located in the structural capsid protein (1 change), and the components of viral replicase complex, the NS3 (2 changes) and NS5 (5 changes) proteins, suggesting a potential role in the capacity of viral infection to vertebrate and/or invertebrate hosts and spreading in the ongoing outbreak.

Financial support: FAPERJ, CNPq, CAPES, FIOCRUZ
+Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

CONTACT US

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco
sala 201, Manguinhos, 21040-900
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

twitterfacebook

SUPPORT PROGRAM

marca fiocruzmarca brasil
marca faperjmarca cnpqmarca capes n