Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 114 | NOVEMBER 2019
Short communication

Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans

Larissa F Paranaiba1, 2, Alessandra A Guarneri2, Ana C Torrecilhas3, Maria N Melo1, Rodrigo P Soares2,+

1Universidade Federal de Minas Gerais, Departamento de Parasitologia, Belo Horizonte, MG, Brasil
2Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Belo Horizonte, MG, Brasil
3Universidade Federal de São Paulo, Departamento de Ciências Farmacêuticas, Diadema, SP, Brasil

DOI: 10.1590/0074-02760190217
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ABSTRACT

The protozoan Trypanosoma cruzi has the ability to spontaneously secrete extracellular vesicles (EVs). In this paper, T. cruzi EVs derived from epimastigote forms were evaluated during interaction with triatomine bugs Rhodnius prolixus and Triatoma infestans. T. cruzi EVs were purified and artificially offered to the insects prior to infection with epimastigote forms. No effect of EVs was detected in the parasite counts in the guts of both vectors after 49-50 days. On the other hand, pre-feeding with EVs delayed parasite migration to rectum only in the gut in R. prolixus after 21-22 days. Those data suggest a possible role of T. cruzi EVs during the earlier events of infection in the invertebrate host.

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Financial support: CNPq, FAPEMIG, FAPESP.
RPS, AAG and MNM are supported by CNPq and FAPEMIG (PPM-00102-16); LFP is supported by FAPEMIG; ACT is supported by FAPESP (2016-01917-3).
+ Corresponding author: rodrigo.pedro@fiocruz.br
 https://orcid.org/0000-0002-7966-3629
Received 26 June 2019
Accepted 22 November 2019

Citation: Paranaiba LF, Guarneri AA, Torrecilhas AC, Melo MN, Soares RP. Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans. Mem Inst Oswaldo Cruz. 2019; 114: e02172019.

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