Mem Inst Oswaldo Cruz, Rio de Janeiro, FAST TRACK
Original Article

Preliminary results of SARS-CoV-2 detection in sewerage system in Niterói municipality, Rio de janeiro, Brazil

Tatiana Prado1, Tulio Machado Fumian1, Camille Ferreira Mannarino2, Adriana Gonçalves Maranhão1, Marilda Mendonça Siqueira3, Marize Pereira Miagostovich1,+

1Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
2Departmento de Saneamento e Saúde Ambiental, Escola Nacional de Saúde Pública Sérgio Arouca, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
3Laboratório de Vírus Respiratório e Sarampo, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil

DOI: 10.1590/0074-02760200196
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ABSTRACT

This study presents preliminary results from a sewage-based surveillance to monitor the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the municipality of Niterói, State of Rio de Janeiro, Brazil. By using ultracentrifugation method associated to RT-qPCR we detected SARS-CoV-2 in 41.6% (5/12) of raw sewage samples obtained from sewage treatment plants and sewers network in the city. This pioneer study carried out in Brazil aims to subsidize information for health surveillance concerning the viral circulation in different areas of the city and, revealed the insertion and importance of Environmental Virology in Health Public Policies.

 

Financial support: This study was supported by Instituto Oswaldo Cruz (PAEF/2), Fundação de Amparo à Pesquisa do Rio de Janeiro (Faperj-E-26/202.821/2018) and CNPq (306655/2018-7).
TP and TMF contributed equally to this work.
+ Corresponding author: marizepm@ioc.fiocruz.br
https://orcid.org/0000-0002-8736-6683

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