Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 115 | MAY 2020
Original Article

Analysing ambiguities in trypanosomatids taxonomy by barcoding

Carolina Boucinha1, Amanda R Caetano1, Helena LC Santos1, Raphael Helaers2, Miikka Vikkula2, Marta Helena Branquinha3, André Luis Souza dos Santos3, Philippe Grellier4, Karina Alessandra Morelli1,5, +, Claudia Masini d‘Avila-Levy1,2, +

1Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Estudos Integrados em Protozoologia,
Coleção de Protozoários da Fiocruz, Rio de Janeiro, RJ, Brasil
2University of Louvain, de Duve Institute, Laboratory of Human Molecular Genetics, Brussels, Belgium
3Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, Brasil
4Muséum National d‘Histoire Naturelle, Unité Molécules de Communication et Adaptation des Microorganisme, Paris, France
5Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcântara Gomes, Departamento de Ecologia, Rio de Janeiro, RJ, Brasil

DOI: 10.1590/0074-02760200504
ABSTRACT

BACKGROUND Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity.
METHODS We sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br.
OBJECTIVE The main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidae genera, to generate phylogenetic trees that can simplify new isolates screenings.
FINDINGS A DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits.
MAIN CONCLUSIONS This survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections.

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Financial support: CNPq, CAPES, FAPERJ.
+ Corresponding authors: davila.levy@ioc.fiocruz.br / karinamorelli@gmail.com
ORCID https://orcid.org/0000-0001-8042-4695
ORCID https://orcid.org/0000-0002-8456-9864
Received 23 January 2020
Accepted 06 May 2020

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