Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 115 | MAY 2020
Original Article

Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata [ACCEPTED ARTICLES / PRELIMINARY VERSION]

Fábio Ribeiro Queiroz1, Laysa Gomes Portilho2, Wander de Jesus Jeremias3, Élio Hideo Babá1, Laurence Rodrigues do Amaral2, 7, Luciana Maria Silva4, Paulo Marcos Zech Coelho1, Roberta Lima Caldeira5, Matheus de Souza Gomes2, 7, +

1Grupo de Pesquisa em Biologia do Schistosoma mansoni e sua Interação com o Hospedeiro, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brasil
2Laboratório de Bioinformática e Análises Moleculares, Universidade Federal de Uberlândia, Campus Patos de Minas, Patos de Minas, Minas Gerais, Brasil
3Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Minas Gerais, Brasil
4Serviço de Biologia Celular do Departamento de Pesquisas e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brasil
5Grupo de Pesquisa em Helmintologia e Malacologia Médica, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brasil
7Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia (RMPCT), Universidade Federal de Uberlândia, Campus Patos de Minas, Patos de Minas, Minas Gerais, Brasil

DOI: 10.1590/0074-02760190498
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ABSTRACT

BACKGROUND Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs have become more relevant. The small RNAs have been considered important through their ability to finely regulate the gene expression in several organisms, thus controlling the functions like cell growth, metabolism, and susceptibility/resistance to infection.
OBJECTIVE The present study aims on identification and characterization of the repertoire of small non-coding RNAs in B. glabrata (Bgl-small RNAs).
METHODS By using small RNA sequencing, bioinformatics tools and RT-qPCR, we identified, characterized, and validated the presence of small RNAs in B. glabrata.
FINDINGS 89 mature miRNAs were identified and five of them were classified as Mollusk-specific. When compared to model organisms, sequences of B. glabrata miRNAs showed a high degree of conservation. In addition, several target genes were predicted for all the mature miRNAs identified. Furthermore, piRNAs were identified in the genome of B. glabrata for the first time. The B. glabrata piRNAs showed strong conservation of uridine as first nucleotide at 5’ end, besides adenine at 10th position. Our results showed that B. glabrata has diverse repertoire of circulating ncRNAs, several which might be involved in mollusk susceptibility to infection, due to their potential roles in the regulation of S. mansoni development.
MAIN CONCLUSIONS Further studies are necessary in order to confirm the role of the Bgl-small RNAs in the parasite/host relationship thus opening new perspectives on interference of small RNAs in the organism development and susceptibility to infection.

+ Corresponding author: matheusgomes@ufu.br
 http://orcid.org/0000-0001-7352-3089
Received 23 December 2019
Accepted 22 May 2020

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