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Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 119 | 2024

Research Articles

Rapid on-site detection of echinococcosis and schistosomiasis based on RPA

Lvbo Tian¹, Ying Shi¹, Yu Yang¹, Yuchen Wang^2,+

¹Sichuan International Travel Health Care Center, Port Epidemic Disease Monitor Key Laboratory of Sichuan Province, Chengdu, Sichuan, China
²Shanghai Customs College, Department of Inspection and Quarantine, Shanghai, China

DOI: 10.1590/0074-02760230244

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ABSTRACT

BACKGROUND Echinococcosis and schistosomiasis, caused by parasitic worms, pose significant threats to millions of people in the world. Rapid and effective pathogen detection and epidemic control by public health authorities are urgently needed.
OBJECTIVES In this study, we aimed to develop rapid on-site detection method to detect echinococcosis and schistosomiasis.
METHODS Recombinase polymerase amplification (RPA) was utilised to examine its efficacy of detection of echinococcosis and schistosomiasis.
FINDINGS The detection probes for RPA were created through comparing parasitic genomes from international genomic data and the sequences generated by our group. We established an optimised RPA on-site testing platform, which significantly reduces the detection time (less than 30 min) and simplifies the operation (free of expensive equipment) as compared to traditional polymerase chain reaction (PCR) method.
MAIN CONCLUSIONS This RPA detection platform in our study for identifying echinococcosis or schistosomiasis pathogens would be greatly applicable for epidemic investigation, border screening, and early clinical diagnosis.

key words: echinococcosis schistosomiasis RPA on-site identification

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