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Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 119 | 2024

Research Articles

First comparative genomics analysis of Corynebacterium auriscanis

Ana Lua de Oliveira Vinhal¹, Max Roberto Batista de Araújo^1,2, Evandro Bento Rodrigues^1,2, Diogo Luiz de Carvalho Castro¹, Carine Rodrigues Pereira³, Dircéia Aparecida Costa Custódio³, Elaine Maria Seles Dorneles³, Flávia Figueira Aburjaile⁴, Bertram Brenig⁵, Vasco Azevedo¹, Marcus Vinicius Canário Viana^1,+

¹Universidade Federal de Minas Gerais, Departamento de Genética, Ecologia e Evolução, Belo Horizonte, MG, Brasil
²Instituto Hermes Pardini-Grupo Fleury, Microbiologia, Núcleo de Operações Técnicas, Vespasiano, MG, Brasil
³Universidade Federal de Lavras, Faculdade de Zootecnia e Medicina Veterinária, Departamento de Medicina Veterinária, Lavras, MG, Brasil
⁴Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil
⁵University of Göttingen, Institute of Veterinary Medicine, Göttingen, Germany

DOI: 10.1590/0074-02760240156

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ABSTRACT

BACKGROUND Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle.
OBJECTIVES The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle.
METHODS The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis.
FINDINGS The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin.
MAIN CONCLUSIONS The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle.

key words: Corynebacterium comparative genomics zoonosis

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