Mem Inst Oswaldo Cruz, Rio de Janeiro, 107(4) June 2012
Primary resistance of HIV to antiretrovirals among individuals recently diagnosed at voluntary counselling and testing centres in the metropolitan region of Recife, Pernambuco
ISetor de Virologia, Laboratório Central de Saúde Pública de Pernambuco, Recife, PE, Brasil
IIPrograma de Pós-Graduação em Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, Brasil
IIICentro de Pesquisa Aggeu Magalhães-Fiocruz, Recife, PE, Brasil
IVCentro de Testagem e Aconselhamento Herbert de Souza,Cabo de Santo Agostinho, PE, Brasil
VLaboratório de Retrovirologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil
Determining the prevalence and type of antiretroviral (ARV) resistance among ARV-naïve individuals is important to assess the potential responses of these individuals to first-line regimens. The prevalence of primary resistance and the occurrence of recent infections among individuals with human immunodeficiency virus (HIV)/acquired immune deficiency syndrome (AIDS) were identified among recently diagnosed patients at five sexually transmitted disease/AIDS testing and counselling centres in the metropolitan region of Recife (RMR), Pernambuco, Brazil, between 2007-2009. One-hundred and eight samples were analysed using the Calypteu00ae BED assay. Males predominated (56%), as did patients aged 31-50 years. Twenty-three percent presented evidence of a recent HIV infection. The median CD4+ T lymphocyte count was 408 cells/mm3 and the median viral load was 3.683 copies/mL. The prevalence of primary resistance was 4.6% (confidence interval 95% = 1-8.2%) based on criteria that excluded common polymorphisms in accordance with the surveillance drug resistance mutation criteria. The prevalence of resistance to non-nucleoside reverse transcriptase, nucleoside/nucleotide reverse transcriptase and protease inhibitors were 3.8%, 1.5% and 0.8%, respectively. Fifty-seven percent of strains were from clade B, 37.7% were clade F and 3.1% were clade C; there were no statistically significant differences with respect to resistance between clades. Recent infection tended to be more common in men (p = 0.06) and in municipalities in the south of the RMR (Jaboatão dos Guararapes and Cabo de Santo Agostinho) (p = 0.046). The high prevalence of recent infection and the high prevalence of non-B strains in this poor Brazilian region merit further attention.u00a0
In Brazil, antiretroviral (ARV) therapy has been offered universally and free of charge to human immunodeficiency virus (HIV)-positive individuals since 1997. Currently, approximately 200,000 people are undergoing ARV treatment. The universal access to ARVs in Brazil has increased survival and improved the quality of life of patients with HIV/acquired immune deficiency syndrome (AIDS) (Teixeira et al. 2004). However, given the sequential use of ARVs in many Brazilian patients and the extensive use of non-boosted protease inhibitors (PIs) in the recent past, it is assumed that a high proportion of patients experience virologic failure and develop ARV resistance, which can lead to the transmission of resistant strains. Initial infection with resistant strains can be related to virologic failure when treatment is started (Gagliani et al. 2011). The likelihood that a transmitted virus may be resistant to at least one ARV varies considerably. This variation is related primarily to the differences in access to therapy (universal versus limited), risk behaviour, compliance with treatment (Paredes et al. 2010) and inclusion of volunteers exposed to ARVs, but not aware of or not willing to disclose this exposure. In USA and Europe, the prevalence of transmitted ARV-resistant strains is between 5-18%; in Asia, this prevalence is 13.8% and in Africa it ranges from 2.2-24% (Ceccherini-Silberstein et al. 2010). There are also regional variations in the prevalence of transmitted ARV resistance over time, with different studies demonstrating stability (Chaix et al. 2009), increases (Wheeler et al. 2010) or decreases in this prevalence (Bracciale et al. 2009). Thus, local studies may play an important role in determining the prevalence of resistance among patients who have never previously taken ARVs, thereby avoiding the initial use of empirically ineffective ARVs and the use of more complex and more expensive ARV schemas than are needed to treat infected individuals in the region.
In Brazil, HIV/AIDS voluntary counselling and testing (VCT) centres have been set up in the municipalities in which 70% of Brazilian AIDS cases are found. With the implementation of VCT centres in Brazil, access to HIV testing has increased and many asymptomatic, infected individuals have sought these centres at earlier stages of the disease to verify their HIV status (Grangeiro 2007).
Investigating the molecular epidemiology of samples from HIV-seropositive individuals who have been diagnosed at VCT centres is of fundamental importance in understanding the details of each localised epidemic in a given region of Brazil.
Recife is the capital of the state of Pernambuco (PE) in the Northeast Region of Brazil. This metropolitan region is an urban area with 3.6 million people (out of a total state population of 7.4 million). The Northeast Region of Brazil is considered less developed than the Southeast and South Regions of the country and the Northeast Region is experiencing an increasing prevalence of AIDS and higher AIDS-related mortality rates than are the southern Regions (MS 2010a). There are nine VCT centres located at strategic points throughout the metropolitan region of Recife (RMR); these VCT centres are located at selected clinics where there is a high demand for HIV testing. The five VCT centres located in the most populous municipalities of the metropolitan region, namely Cabo de Santo Agostinho, Jaboatão dos Guararapes, Olinda, Paulista and Recife, account for the largest number of patients tested for HIV-1 in the RMR. Most of these centres have specialised outpatient clinics funded primarily by resources from the Department of Sexually Transmitted Disease (STD)/AIDS and Viral Hepatitis of the Brazilian Ministry of Health (MS 2010b).
In this study we sought (i) to determine the prevalence of primary resistance and recent infection among HIV-1-seropositive individuals who had been recently diagnosed at the five most important VCT centres in the RMR, (ii) to compare the characteristics of individuals with recent and established infections and (iii) to analyse the socio-demographic characteristics of individuals with HIV-1 subtypes B and F.
SUBJECTS, MATERIALS AND METHODS
The users of the VCT centres selected for this study (Cabo de Santo Agostinho, Jaboatão dos Guararapes, Olinda, Paulista and Recife) were diagnosed as HIV positive by two different ELISA assays and one positive confirmatory test (immunofluorescence or western blot assay). When these individuals returned to the centres to receive their results, they were informed of this study during the post-test counselling. Those individuals who agreed to participate signed the informed consent document and then blood was collected for HIV characterization and for the Calypte BED assay. Along with the specific form for the project, the samples were sent to the Virology Sector of Central Public Health Laboratory (LACEN)-PE and stored at -70ºC.
Between July 2007-March 2009, the partial HIV genomes from 130 samples from individuals who had recently been diagnosed with HIV-1 and had never received ARV treatment were sequenced.
Viral RNA was extracted using the QIAamp® RNA Mini Kit (Qiagen, Germany). For reverse transcription of the RNA to yield cDNA, polymerase chain reaction amplification followed by genetic sequencing of the product was performed using the TRUGENE® HIV-1 Genotyping Assay (Siemens Diagnostics, USA). This genotyping assay detects genomic HIV mutations in the protease (codons 1-99) and the reverse transcriptase (codons 10-297) regions of the pol gene.
The genetic sequences generated were analysed using the OpenGene® DNA Sequencing System (Siemens Diagnostics, USA). ARV resistance mutations and subtypes were submitted to the Stanford HIV Drug Resistance Database (hivdb.stanford.edu) and the subtypes were further confirmed using SIMPLOT version 2. The mutations were identified in accordance with the surveillance drug resistance mutation (SDRM) criteria, which exclude common polymorphisms in the evaluation of transmitted ARV-resistant HIV (Bennett et al. 2009). The Calypte® BED assay (Calypte Biomedical Corporation) was performed in accordance with the manufacturer's instructions for the serological differentiation of recent seroconverters and long-term seroconverters.
The patients' socio-demographic and laboratory data were retrieved from the sample referral records sent from the VCT centre to the Virology Sector of LACEN-PE based on the user history provided by the Laboratory Test Result System database. The viral load and CD4+ T lymphocyte count were retrieved from Brazilian System of Laboratory Examinations Control. The Logistics Control System of Medicines (SICLOM), managed by the State Coordination of STD and AIDS of PE, was consulted to identify patients with prior exposure to ARVs among those patients whose viruses harboured resistance mutations.
Data were analysed using STATA version 9.0. Descriptive statistics are presented as percentages, medians and percentiles. The Kolmogorov-Smirnov test for normality was applied to the CD4 count and viral load data and the null hypothesis was rejected; thus, the nonparametric Mann-Whitney test was used. We used the chi-squared test to compare proportions and we used Fisher's exact test when necessary. Statistical significance was set at the 5% level.
The study was approved by the Ethical in Research Committee of the Federal University of Pernambuco (protocol CEP/CCS/UFPE 120/07).
The patients' characteristics are shown in Table I. Samples were available from 108 individuals for testing for recent HIV infection and this testing revealed a prevalence of recent infection of 23.1% [confidence interval (CI) 95% 14.9-31.3%]. The prevalence of primary resistance was 6.1% (CI 95% 1.6-10.6%) based on International AIDS Society (IAS) (2008) (Johnson et al. 2008) table of mutations and 4.6% (CI 95% 1.0-8.2%) based on the SDRM criteria (2009) (Bennett et al. 2009). Subtype B was the most prevalent (56.9%) in the study population, followed by F (37.7%), C (3.1%) and B/F (2.3%).
The mutations associated with resistance to reverse transcriptase inhibitors and PIs that were found among eight patients in the study are detailed in Table II, which also presents the socio-demographic and laboratory data of these patients.
The percentage of patients with recent infection varied among the studied municipalities. The prevalence of recent infection was greater among patients from the city of Recife and the southern region of the RMR (Jaboatão dos Guararapes and Cabo de Santo Agostinho), whereas the prevalence of chronic infection was greater among those patients from the northern region of the RMR (Olinda and Paulista). There was a trend towards a higher prevalence of recent infections among men (Table III).
There were no statistically significant differences with respect to socio-demographic and laboratory characteristics between individuals with and without resistance or between individuals infected by the B and F subtypes of HIV-1 (Table IV).
The prevalence of primary resistance to ARVs (considering only reverse transcriptase inhibitors and PIs) observed in this study was 4.6% (CI 95% 1-8.2%) when using the SDRM criteria, which exclude common polymorphisms (Bennett et al. 2009).
Due to the high cost of monitoring ARV resistance by means of genotyping, the World Health Organization recommends that the surveillance of resistance in small geographical areas should be stratified by applying a sequential sampling method known as threshold surveying of HIV resistance, thus enabling the use of a small set of samples (n < 50). This method classifies HIV resistance into three categories of prevalence: low (< 5%), moderate (5-15%) and high (> 15%) (Myatt et al. 2008).
In Brazil, two major studies on the prevalence of primary HIV drug resistance were published in 2009. The first included 400 participants from 13 Brazilian cities. It is worth noting that only one centre, in Salvador (in the Northeast Region), was located outside of the South and Southeast Regions and that 220 samples originated from the state of São Paulo (SP). Using the IAS criteria, the percentage of HIV-positive individuals whose viruses harboured resistance mutations was 5.7% (Sprinz et al. 2009). In the present study, using samples from the RMR, the observed prevalence of resistance mutations was determined to be 6.1% when using the IAS criteria; this prevalence is very similar to that reported by Sprinz et al. (2009).
One evaluation carried out at a national level was the study of the National Network for the Surveillance of Drug Resistance in Brazil (HIV-BResNet), which results were published in 2009. This study included 210 recently diagnosed individuals from six state capitals in Brazil, of which two capitals were in the Southeast Region (São Paulo and Rio de Janeiro), one in the South Region (Porto Alegre), one in the Central-West Region (Brasília), one in the North Region (Belém) and one in the Northeast Region (Salvador). The individuals were classified according to HIV mutations based on the SDRM criteria. In this study, 8.1% of the isolates harboured resistance mutations. The levels of resistance in São Paulo, Rio de Janeiro and Belém were intermediate (between 5-15%), whereas the levels were low (< 5%) in Brasília, Porto Alegre and Salvador (Inocêncio et al. 2009).
Using the SDRM criteria, the present study showed that 4.6% of the strains had resistance mutations; this result coincides with the low resistance prevalence that has been detected outside of the Southeast Region, which is the epicenter of the HIV epidemic in Brazil (Inocêncio et al. 2009). These results are also similar to the results of a study conducted in Recife in 2002 that showed that 3.6% of HIV-positive patients were infected with viruses that harboured resistance-related mutations (Medeiros et al. 2006).
Regarding the Northeast Region, data on the resistance of HIV to drugs in the states of Bahia and Ceará (CE) have recently been published. The HIV BResNet study showed that there was a low rate of resistance to drugs - less than 5% - in Salvador (Inocêncio et al. 2009). A study conducted in CE found an intermediate rate of resistance, 9.5% (Arruda et al. 2010), which was higher than the rates of resistance found in the two major Brazilian studies that included states outside of the Southeast Region. Given the differences in the data among these studies, in addition to the similarities among and the proximity of the three northeastern capitals, the precise composition of the populations in each study needs to be determined.
According to the analysis in the present study using the SDRM criteria, 3.84% of the mutations observed were associated with non-nucleoside reverse-transcriptase inhibitors (NNRTIs), 1.53% with NRTIs and 0.77% with PIs; this profile is similar to the profiles observed recently in Brazil (Inocêncio et al. 2009, Sprinz et al. 2009) and elsewhere (Little et al. 2001).
From the analysis of the frequency of mutations associated with each class of ARVs, we found that there were more mutations associated with NNRTIs, a finding that was absent from an earlier study carried out in Recife in 2002 (Medeiros et al. 2006) and from a nationwide study (Brindeiro et al. 2003).
Regarding NRTIs, unlike in other studies, in this study two of our samples contained viruses with the 184V mutation and only one sample contained a virus with the 215F mutation. It is important to emphasize that the 184V mutation was found in the viruses of two individuals with recent infections, which may explain the presence of this mutation because there is a trend for this mutation to become undetectable over time among individuals infected with ARV-resistant strains (Jain et al. 2011).
The present study revealed a high prevalence of recent infections (23%), but there were no significant differences in the prevalence of mutations between the recently and chronically infected populations (Fisher's exact test, p = 0.327). Two samples from patients with recent infections and two samples from patients with chronic infections contained viruses that harboured NNRTI-related mutations, whereas NRTI-related mutations were found only in the viruses from the recently infected group (in 2 samples). The only sample with a mutation related to a PI was found among the patients with chronic infections. These findings most likely reflect the greater circulation of NNRTI-resistant strains, owing to the fact that NNRTIs are generally used in first-line regimens in Brazil (MS 2008).
Recent infections tended to be more common in men, in the municipality of Recife and in the southern part of the RMR (Jaboatão dos Guararapes and Cabo de Santo Agostinho). It should be noted that the severity of the AIDS epidemic continues to increase in the Northeast Region of Brazil. Recife currently ranks as the state capital with the fifth highest number of new cases of AIDS in Brazil, with a detection rate of 58.4 cases/100,000 persons in 2010 (MS 2010a). Regarding the southern part of the RMR, the larger number of recent infections is most likely due to the presence of the tourist resort of Porto de Galinhas and the industrial Port Complex of Suape, which is the new centre of economic development in the region. The availability of jobs at the resort and the port has resulted in the migration of young workers, some of them returning from the Southeast Region of Brazil (the epicenter of the AIDS epidemic in Brazil) back to their place of origin (IBGE, available from: ibge.gov.br/home/estatistica/populacao/reflexoes_deslocamentos/ deslocamentos.pdf).
It should be noted that the patient sample included in the present study, which was exclusively recruited at VCT centres from among individuals recently diagnosed HIV, exhibited a prevalence of recent infection of 23%. The results for this sample may more accurately reflect the percentage of recent HIV infections in the general HIV-infected population in this region than the results for samples from studies conducted at hospitals that provide specialized care. We thus verified that over 75% of the infections had been present for more than six months, thereby increasing the likelihood of viral transmission. These results demonstrate the importance of more actions that are aimed at prevention and of easier access to HIV testing, particularly in the areas where the diagnosis was shown to have been made at a late stage, such as in the northern part of the RMR. However, a similar proportion of recent HIV infections was found by Puchhammer-Stöckl et al. (2005) among newly diagnosed cases of HIV infection in Austria; this population is considered to be knowledgeable about HIV transmission and had easy access to diagnosis and widely available ARV treatment. Another study that compared the immune status at presentation for HIV clinical care among two different cohorts from Rio de Janeiro and Baltimore demonstrated that more than 50% of the patients presented with CD4 counts < 350 cells/mm3 and 75% presented with viral loads > 10,000 copies/mm3 (Moreira et al. 2011). These findings reinforce the hypothesis that even in a population that is well informed about HIV, individual awareness of the risk of infection is still low.
As has been observed in all Regions of Brazil, except the South, there is a predominance of HIV subtype B. However, in the present study, a high percentage of subtype F (37.7%) was detected in the RMR. This rate was much higher than that generally observed in other regions of the country (Inocêncio et al. 2009). In addition, it appears that there may be a trend towards an increase in the circulation of subtype F in this particular Region; the prevalence of this subtype was already high (26%) in Recife in 2002 according to a previous study (Medeiros et al. 2006). The prevalence of subtype F was 22% in another study carried out between 2002-2004 using samples of patients with secondary resistance in PE (Cavalcanti et al. 2007). An epidemic of B/F recombinant strains that include circulating recombinants forms 28 and 29 in approximately 50% of infected individuals was detected in the port city of Santos in the Southeast Region, where there are also high levels of transmitted resistance (Sucupira et al. 2007, Teixeira et al. 2010).
It is interesting to note that although subtype B is predominant in Brazil, there is a trend towards an increasing prevalence of subtype C in the South and Southeast Regions of the country. In a recent study carried out in six Brazilian state capitals with ARV-naïve patients, Inocêncio et al. (2009) observed a rate of 69% of subtype C in Rio Grande do Sul; this rate is much higher than that found by Brígido et al. (2007). In addition, Brígido et al. (2011) discovered an increased presence of subtype C in SP. It is of note that resistance-related mutations may vary among different subtypes in Brazil (Munerato et al. 2010) and studies targeting non-B strains certainly merit further attention.
We recognize that the small number of samples and the small size of the genomic region of HIV characterized in this study may preclude more definitive conclusions. In addition, a further limitation was the sampling bias; a smaller number of samples from Recife was analyzed despite its being the largest city in the region. This sampling bias precluded making more comparisons between the isolates. However, we found that there is an increased prevalence of subtype F in this region.
Transmitted resistance is still low, but the profile of transmitted mutations may be changing in this region. Future trends need to be carefully observed and evaluated.
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