Mem Inst Oswaldo Cruz, Rio de Janeiro, 96(7) October 2001
Short communication

Black Flies (Diptera: Simuliidae) of French Guiana: Cytotaxonomy and a Preliminary List of Species

Neusa Hamada
+, Florence Fouque*

Coordenação de Pesquisas em Entomologia, Instituto Nacional de Pesquisas da Amazônia, Caixa Postal 478, 69011-970 Manaus, AM, Brasil
*Laboratoire d'Entomologie Médicale, Institut Pasteur de la Guyane, Cayenne, French Guiana

Page: 955-959
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ABSTRACT

The objectives of the present study were to broaden the survey of simuliid species in French Guiana and to cytologically analyze the species in theu00a0Simulium perflavumu00a0species group. Twelve species ofu00a0Simuliumu00a0were collected from whichu00a0S. goeldii, S. quadrifidum, S. trombetense, S. near incrustatum, S. metallicum(s.l.)u00a0sp1, S. metallicumu00a0(s.l.)u00a0sp2 andu00a0S. ochraceum(s.l.)u00a0are reported for the first time for this region. The only species collected in theu00a0S. perflavumu00a0group was S. rorotaense; 34 larvae of this species were cytologically analyzed, all of which had the standard sequence.u00a0S. metallicum (s.l.),u00a0S. ochraceum(s.l.),u00a0S. guianense (s.l.)u00a0andu00a0S. oyapockense (s.l.)u00a0are involved with transmission of onchocerciasis in Central and South America, however, in French Guiana these species were not found biting humans during the sampling period. With the few collections made during this study, we increase the number of simulid species known in French Guiana from 6 to 13. It is clear that more simuliid species can be expected to be found when more sampling is done, including collections in other ecoregions in French Guiana.

Black flies have received little attention in French Guiana, where the last studies on these insects were those of Floch and Abonnenc in the 40s. These authors reported Simulium cauchense Floch & Abonnenc, Simulium rorotaense Floch & Abonnenc, Simulium maroniense Floch & Abonnenc, Simulium iracouboense Floch & Abonnenc, Simulium oyapockenseFloch & Abonnenc and Simulium guianense Wise (Floch & Abonnenc 1946a,b). S. maroniense was made synonymous with S. rorotaense by Shelley et al. (1984, 1997); however, Hamada and Adler (1998, 1999) studying specimens collected in Brazil found morphological and chromosomal evidence demonstrating that they are really two distinct species.

The objectives of the present study were to broaden the survey of simuliid species in French Guiana and, since polytene chromosomes of S. rorotaense and S. maroniense had previously not been examined from their type localities, to determine if the chromosome patterns of these species from the type locality correspond to the defined standards.

Larvae and pupae were collected in June 1999 around Cayenne, Saül, Vovony community (Approuage River), Maroni River (on the border with Surinam) and along the highway that connects Cayenne to Iracoubo (Fig. 1Table). Specimens were hand collected and preserved in Carnoy (1 part absolute ethanol: 3 parts glacial acetic acid). Pupae with pharate adults were, whenever possible, reared in plastic vials with wet filter paper to confirm larval and pupal identifications; adults emerged from reared pupae were mounted on triangles or preserved in 80% ethanol. Specimens for chromosome analyses were dissected and stained with the Feulgen technique (Rothfels & Dunbar 1953). Cytological comparisons were made using the standard maps established by Hamada and Adler (1999).

Sixteen samples were collected in French Guiana (Table). Twelve species of Simulium were collected: S. cauchense, S. guianense; S. iracouboenseS. oyapockenseS. rorotaense; the following species are first reported for this country: S. goeldii Cerqueira & Nunes de Mello, S. quadrifidum Lutz, S. trombetense Hamada, Py-Daniel & Adler, S. nearincrustatum (Figs 12-14), S. metallicum (s.l.) sp1 (Figs 2-48), S. metallicum (s.l.) sp2 (Figs 5-7) and S. ochraceum (s.l.) (Figs 9-11). Voucher specimens were deposited at Invertebrate Collection, INPA, Manaus, Brazil and Medical Entomology Laboratory, Institut Pasteur, Cayenne, French Guiana.

Of the species previously reported for French Guiana only S. maroniense was not collected during the sampling period; we were unable to locate its type locality (Coeur Maroni stream-Sinnamary). Apparently this stream was located in an area that now is inundated due to the construction of a hydroelectric dam (Barrage de Petit-Saut). Therefore, only S.rorotaense was examined at the cytological level. Larvae of S. rorotaense from its type locality (Rorota, Montagne du Mahury, Table) were examined; 67 last-instar larvae were stained (27 males and 40 females), of which 50.7% were completely analyzed (n = 34). All completely analyzed specimens had the standard sequence; the only polymorphism observed was the Heteroband 1 (Hb1), also found in populations of S. rorotaenseS. maroniense and S. trombetense in the Brazilian Amazon region (Hamada & Adler 1999).

In the two large rivers sampled (Maroni and Approuage) the most abundant species was S. iracouboense; this species also occurred in smaller streams (Table). S. guianense (s.l.) and S. oyapockense (s.l.) were restricted to these two large rivers, but in very low density. Although S. guianense (s.l.) and S. oyapockense (s.l.) are involved with transmission of onchocerciasis in Brazil and Venezuela (Shelley et al. 1997, Grillet et al. 2000), in French Guiana these species appear not to be anthropophilic, since the indigenous people who live along these water courses did not complain about their biting. S. metallicum cytotype E is involved in onchocerciasis transmission in mountainous areas of Venezuela, near the Caribbean coast (Grillet et al. 1995). This nominal species includes at least 12 cytotypes (Conn et al. 1989, Arteaga & Muñoz de Hoyos 1999). We collected two morphotypes that by larvae and gill filament morphology (Figs 4 - 9) can be placed in the S. metallicum species complex; one of these (S. metallicum sp2) may be Simulium horacioi Okazawa & Onishi, which corresponds to Conn et al. (1989) cytospecies H. Pupae of this morphotype were not collected, but dissected gill histoblasts of last-instar larvae indicate that it has typical S. metallicum (s.l.) gill morphology. Both mor-photypes were collected in small (first-order) mountain streams at less than 300 m altitude; they were collected syntopically in one stream (Table). These morphotypes also seem not to be anthropophilic because, when asked, people living along the river do not complain about anthropophilic black flies in the area. Other researchers have reported S. metallicum (s.l.) at higher altitudes and, in Venezuela and Colombia they were reported as anthropophilic (Grillet et al. 1995, Arteaga & Muñoz de Hoyos 1999).

The S. ochraceum species complex is known to include at least two cytospecies and one cytotype; cytospecies A is highly anthropophilic and is involved with onchocerciasis transmission in Guatemala and Mexico (Millest 1992, Hirai et al. 1994). In Venezuela, S. ochraceum (s.l.) co-occurs with S. metallicum cytotype E and is not involved with onchocerciasis transmission (Grillet & Barrera 1997). S. ochraceum (s.l.) was not found biting humans at the breeding sites, and it occupied a different habitat from the populations studied by Hirai et al. (1994) and Grillet and Barrera (1997), who reported this species above 600 m altitude. The five sites (Table) with this species were small, pristine, first-order streams, located in forested areas at less than 300 m altitude, and temperatures of 24-25ºC.

Simulium near incrustatum (Fig. 2) was collected in the larval and pupal stages in two small streams. Confirmation of this identification may only be made after rearing pupae to obtain the adults.

With the few collections made during this study, we increase the number of simuliid species known in French Guiana from 6 to 13. It is clear that more simuliid species can be expected to be found when more sampling is done, including collections in other ecoregions in French Guiana. Cytological and biological studies are also needed, especially on S. metallicum (s.l.), S. ochraceum (s.l.), S. guianense (s.l.) and S. oyapockense (s.l.), since some of the cytotypes or cytospecies in these nominal species are involved in onchocerciasis transmission in Central and South America.

 

ACKNOWLEDGEMENTS

To Dr Jean-Louis Sarthou, Director of Pasteur Institute of French Guiana, Cayenne, for the support provided. To Stéphane Laventure, Romuald Carinci, Jean Issaly, and Pascal Gaborit from the Pasteur Institute, for field support. Benedito Otávio L Almeida drew the illustrations and Jorge Dácio inked the map. Philip M Fearnside reviewed the manuscript.

 

REFERENCES

Arteaga L, Muñoz de Hoyos P 1999. New cytotype in the Simulium metallicum complex (Diptera: Simuliidae) from Cundinamarca, Colombia. J Med Entomol 36: 133-140.

Conn J, Rothfels KH, Procunier WS, Hirai H 1989. The Simulium metallicum species complex (Diptera: Simuliidae) in Latin America: a cytological study. Can J Zool 67: 1217-1245.

Floch H, Abonnenc E 1946a. Simulides de la Guyane Française I. S. guianense Wise 1911, S. rorotaense n. sp.,S. maroniense n. sp. Publ Inst Pasteur Guyane Franc et Terr de l'Inini 136: 1-20.

Floch H, Abonnenc E 1946b. Simulides de la Guyane Française (II). S. cauchense n. sp., S. oyapockense n. sp.,S. iracouboense n. sp. Publ Inst Pasteur Guyane Franc et Terr de l'Inini 137: 1-19.

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Grillet ME, Barrera R, Conn J 1995. Simulium metallicum cytospecies E larval habitat characterization in the Altamira focus of onchocerciasis, northern Venezuela. Med Vet Ent 9: 195-201.

Grillet ME, Basáñez MG, Villamizar NJ, Frontado H, Cortez J, Coronel P, Escalona M, Botto C 2000. Aspects of the vectorial competence of onchocerciasis vectors in the Amazonian focus of southern Venezuela before and during invermectin distribution. The British Simulium Group Bull 15: 8-10.

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Hamada N, Adler PH 1999. Cytotaxonomy of four species in the Simulium perflavum species-group (Diptera: Simuliidae) from Brazilian Amazonia. Syst Entomol 24: 273-288.

Hirai H, Procunier WS, Ochoa JO, Uemoto K 1994. A cytogenetic analysis of the Simulium ochraceum species complex (Diptera: Simuliidae). Genome 37: 36-53.

Millest AL 1992. Identification of members of the Simulium ochraceum species complex in the three onchocerciasis foci in Mexico. Med Vet Ent 6: 23-28.

Rothfels KH, Dunbar TW 1953. The salivary gland chromosomes of the black fly Simulium vittatum Zett. Can J Zool 31: 226-241.

Shelley AJ, Luna Dias APA, Maia-Herzog M 1984. New specific synonymy in Netropical Simulium s.l. (Diptera: Simuliidae). Mem Inst Oswaldo Cruz 79: 143-161.

Shelley AJ, Lowry CA, Maia-Herzog M, Luna Dias APA, Moraes MAP 1997. Biosystematic studies on the Simuliidae (Diptera) of the Amazonia onchocerciasis focus. Bull Nat Hist Mus Lond (Ent.) 66: 1-121.

+Corresponding author. Fax: +55-92-642.8909. E-mail:u00a0nhamada@inpa.gov.br
Received 2 February 2001
Accepted 9 May 2001

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