Mem Inst Oswaldo Cruz, Rio de Janeiro, 112(7) July 2017
Short communication

Participation of the arcRACME protein in self-activation of the arc operon located in the arginine catabolism mobile element in pandemic clone USA300

Zayda Lorena Corredor Rozo1, Ricaurte Alejandro Márquez-Ortiz1, Betsy Esperanza Castro1, Natasha Vanegas Gómez1,2, Javier Escobar-Pérez1,+

1Universidad El Bosque, Bacterial Molecular Genetics Laboratory, Bogotá, Colombia
2University of Technology, Faculty of Science, I3 Institute, Infection, Immunity and Innovation, Sydney, Australia

Page: 499-503 DOI: 10.1590/0074-02760160424
800 views 867 downloads

Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an arginine catabolism mobile element (ACME) carrying another such cluster, which gives this clone advantages in colonisation and infection. Gene arcR, which encodes an oxygen-sensitive transcriptional regulator, is inside ACME and downstream of the constitutive arc gene cluster, and this situation may have an impact on its activation. Different relative expression behaviours are proven here for arcRACME and the arcACME operon compared to the constitutive ones. We also show that the artificially expressed recombinant ArcRACME protein binds to the promoter region of the arcACME operon; this mechanism can be related to a positive feedback model, which may be responsible for increased anaerobic survival of the USA300 clone during infection-related processes.

Financial support: Departamento Administrativo de Ciencia, Tecnología e Innovación COLCIENCIAS (grant 1308-51929126); ZLCR was supported by the COLCIENCIAS (grant 1308-51929126).
+ Corresponding author:
Received 19 September 2016
Accepted 4 March 2017

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