Mem Inst Oswaldo Cruz, Rio de Janeiro, 112(9) September 2017
Original Article

Specific antigen serologic tests in leprosy: implications for epidemiological surveillance of leprosy cases and household contacts

Ana Paula Mendes Carvalho1,+, Angélica da Conceição Oliveira Coelho2, Rodrigo Correa-Oliveira1,3, Francisco Carlos Félix Lana4

1Universidade Federal de Minas Gerais, Escola de Enfermagem, Programa de Pós-Graduação em Enfermagem, Belo Horizonte, MG, Brasil
2Universidade Federal de Juiz de Fora, Faculdade de Enfermagem, Departamento de Enfermagem Básica, Juiz de Fora, MG, Brasil
3Fundação Oswaldo Cruz-Fiocruz, Centro de Pesquisas René Rachou, Laboratório de Imunologia Celular e Molecular, Belo Horizonte, MG, Brasil
4Universidade Federal de Minas Gerais, Escola de Enfermagem, Departamento de Enfermagem Materno-Infantil e Saúde Pública, Programa de Pós-Graduação em Enfermagem, Belo Horizonte, MG, Brasil

Page: 609-616 DOI: 10.1590/0074-02760160505
680 views 444 downloads

BACKGROUND There is a lack of straightforward tests for field application and known biomarkers for predicting leprosy progression in infected individuals.

OBJECTIVE The aim was to analyse the response to infection by Mycobacterium leprae based on the reactivity of specific antigens: natural disaccharide linked to human serum albumin via an octyl (NDOHSA), a semisynthetic phenolic glycolipid-I (PGL-I); Leprosy Infectious Disease Research Institute Diagnostic-1 (LID-1) and natural disaccharide octyl - Leprosy Infectious Disease Research Institute Diagnostic-1 (NDOLID).

METHODS The study population consisted of 130 leprosy cases diagnosed between 2010 and 2015 and 277 household contacts. An enzyme-linked immunosorbent assay (ELISA) was used to analyse the reactivity of antibodies against NDOHSA, LID-1 and NDOLID. The samples and controls were tested in duplicate, and the antibody titer was expressed as an ELISA index. Data collection was made by home visits with application of questionnaire and dermatological evaluation of all household contacts to identify signs and symptoms of leprosy.

FINDINGS Significant differences in the median ELISA results were observed among leprosy cases in treatment, leprosy cases that had completed treatment and household contacts. Higher proportions of seropositivity were observed in leprosy cases in treatment. Seropositivity was also higher in multibacillary in relation to paucibacillary, with the difference reaching statistical significance. Lower titers were observed among cases with a longer treatment time or discharge. For household contacts, the differences according to the clinical characteristics of the leprosy index case were less pronounced than expected. Other factors, such as the endemicity of leprosy, exposure outside the residence and genetic characteristics, appeared to have a greater influence on the seropositivity.

MAIN CONCLUSIONS Serologic tests could be used as auxiliary tools for determining the operational classification, in addition to identifying infected individuals and as a strategy for surveillance of household contacts.

Financial support: FAPEMIG, Ministério da Saúde/Secretaria de Vigilância em Saúde.
+ Corresponding author:
Received 20 November 2016
Accepted 15 May 2017

Our Location

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco 
sala 201, Manguinhos, 21040-900 
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This email address is being protected from spambots. You need JavaScript enabled to view it.

Support Program


fiocruz governo
faperj cnpq capes