Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 114 | NOVEMBER 2019
Original Article

Acinetobacter baumannii infections in Amazon Region driven by extensively drug resistant international clones, 2016-2018

Raquel Vosges Caldart1, Erica L Fonseca2,+, Fernanda Freitas2, Luiza Rocha3, Ana Carolina Vicente2

1Universidade Federal de Roraima, Boa Vista, RR, Brasil
2Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Genética Molecular de Microrganismos, Rio de Janeiro, RJ, Brasil
3Laboratório Central de Saúde Pública de Roraima, Boa Vista, RR, Brasil

DOI: 10.1590/0074-02760190232
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ABSTRACT

BACKGROUND Acinetobacter baumannii is a leading cause of nosocomial infections. This species is characterised by the presence of pandemic lineages (International Clones) that present a broad antimicrobial resistance profile.

OBJECTIVE To perform the molecular epidemiology of carbapenem-resistant A. baumannii from a clinical setting in the Amazon Basin, and to characterise their antimicrobial resistance determinants.

METHODS The genetic relationship of carbapenem-resistant A. baumannii were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Class A, B and D β-lactamase genes were screened by polymerase chain reaction (PCR) and sequencing. The antimicrobial susceptibility profile was obtained by Disc-diffusion method and minimum inhibitory concentration (MIC) determination.

FINDINGS All carbapenem-resistant A. baumannii strains belonged to three international clones, IC-1, IC-5 and IC-6, the latter recently reported by the first time in Brazil. The major determinant of carbapenem resistance in IC-1 and IC-5 strains was blaOXA-23, associated with ISAba1 and ISAba3, respectively, while IC-6 harboured the blaOXA-72.

CONCLUSIONS The A. baumannii epidemiology in Brazilian Amazon Region was unknown. It was demonstrated that A. baumannii XDR international clones were responsible for nosocomial infections in Boa Vista during 2016-2018, revealing that the epidemiological scenario of A. baumannii infections in Amazon Region resembles those from the cosmopolitan regions worldwide.

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Financial support: CNPq, Instituto Oswaldo Cruz (grant).
+ Corresponding author: ericafon@ioc.fiocruz.br
https://orcid.org/0000-0002-5029-7389
Received 04 July 2019
Accepted 06 November 2019

Citation: Caldart RV, Fonseca EL, Freitas F, Rocha L, Vicente AC. Acinetobacter baumannii infections in Amazon Region driven by extensively drug resistant international clones, 2016-2018. Mem Inst Oswaldo Cruz. 2019; 114: e190232.

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