Mem Inst Oswaldo Cruz, Rio de Janeiro, VOLUME 114 | NOVEMBER 2019
Short communication

Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)

Magdalena Laurito1,2,+, Iliana M Ontivero1, Walter R Almirón1,2

1Universidad Nacional de Córdoba, Centro de Investigaciones Entomológicas de Córdoba, Facultad de Ciencias Exactas,  Físicas y Naturales, Córdoba, Argentina
2Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Investigaciones Biológicas y Tecnológicas, Córdoba, Argentina

DOI: 10.1590/0074-02760190208
23 views 0 downloads
ABSTRACT

Sand fly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand flies of the New World.

REFERENCES
01. WHO - World Health Organization. Control of the leishmaniases: report of a meeting of the WHO Expert Commitee on the Control of Leishmaniases. World Health Organization Technical Report Series. 2010; 949: 22-6.
02. Salomón OD, Rosa JR, Stein M, Quintana MG, Fernández MS, Visintin AM, et al. Phlebotominae (Diptera: Psychodidae) fauna in the Chaco region and Cutaneous Leishmaniasis transmission patterns in Argentina. Mem Inst Oswaldo Cruz. 2008; 103(6): 578-84.
03. Salomón OD, Quintana MG, Rosa JR. Ecoepidemiología de la Leishmaniasis cutánea en la Argentina. Salud(i)Ciencia. 2008; 16(5): 514-20.
04. Visintin AM, Beranek MD, Amieva MJ, Rosa JR, Almirón WR, Salomón OD. Spread of Phlebotominae in temperate climates: province of Córdoba, Argentina. Mem Inst Oswaldo Cruz. 2016; 111(1): 75-8.
05. Ontivero IM, Beranek MD, Rosa JR, Ludueña-Almeida FF, Almirón WR. Seasonal distribution of phlebotomine sandfly in a vulnerable area for tegumentary leishmaniasis transmission in Córdoba, Argentina. Acta Trop. 2018; 178: 81-5.
06. Galati EAB. Morfologia, terminologia de adultos e identificação dos táxons da América. In: Rangel EF, Lainson R, orgs. Flebotomíneos do Brazil. Rio de Janeiro: Fiocruz; 2003. 53-175.
07. Szelag EA, Rosa JR, Galati EA, Andrade Filho JD, Salomón OD. Considerations on the species complex of the Cortelezzii series (Diptera: Psychodidae) and description of Evandromyia chacuensis sp. nov., a new Phlebotomine species of the Chaco Region, Argentina. J Med Entomol. 2018; 55(4): 902-9.
08. Hebert PDN, Cywinska A, Ball SL, de Waard JR. Biological identifications through DNA barcodes. Proc R Soc Lond B Biol Sci. 2003; 270(1512): 313-21.
09. Hoyos-López R, Uribe S, Vélez I. [Typification of Colombian specimens of Lutzomyia longipalpis (Diptera: Psychodidae) by “Barcoding”]. Rev Colomb Entomol. 2012; 38(1): 134-40.
10. Pinto IDS, Chagas BD, Rodrigues AAF, Ferreira AL, Rezende HR, Bruno RV, et al. DNA barcoding of neotropical sand flies (Diptera, Psychodidae, Phlebotominae): species identification and discovery within Brazil. PLoS One. 2015; 10(10): e0140636.
11. Rodrigues BL, Carvalho-Costa LF, Pinto IDS, Rebêlo JMM. DNA barcoding reveals hidden diversity of sand flies (Diptera: Psychodidae) at fine and broad spatial scales in Brazilian endemic regions for leishmaniasis. J Med Entomol. 2018; 55(4): 893-901.
12. Beeble NW. DNA barcoding mosquitoes: advice for potential prospectors. Parasitology. 2018; 145(5): 622-33.
13. Puillandre N, Lambert A, Brouillet S, Achaz G. ABGD, automated barcode gap discovery for primary species delimitation. Mol Ecol. 2012; 21: 1864-77.
14. Marcondes CB. A proposal of generic and subgeneric abbreviations for phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) of the world. Entomol News. 2007; 118(4): 351-7.
15. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994: 3: 294-9.
16. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series. 1999; 41(41): 95-8.
17. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004; 32(5): 1792-7.
18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16(2): 111-20.
19. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016; 33(7): 1870-4.
20. Avise JC. Phylogeography: the history and formation of species. Cambridge: Harvard University Press; 2000.
21. Coyne JA, Orr HA. Speciation. Sunderland: Sinauer Associates, Inc; 2004.
22. Salomón OD, Quintana MG, Bezzi G, Morán ML, Betbeder E, Valdéz DV. Lutzomyia migonei as putative vector of visceral leishmaniasis in La Banda, Argentina. Acta Trop. 2010; 113(1): 84-7.
23. Moya SL, Giuliani MG, Acosta MM, Salomón OD, Liotta DJ. First description of Migonemyia migonei (França) and Nyssomyia whitmani (Antunes & Coutinho) (Psychodidae: Phlebotominae) natural infected by Leishmania infantum in Argentina. Acta Trop. 2015; 152: 181-4.
24. Rosa JR, Pereira P, Brazil RP, Andrade Filho JD, Salomón OD, Szelag EA. Natural infection of Cortelezzii complex (Diptera: Psychodidae: Phlebotominae) with Leishmania braziliensis in Chaco, Argentina. Acta Trop. 2012; 123(2): 128-31.

Financial support: Agencia Nacional de Ciencia y Técnica, Financial support: Agencia Nacional de Ciencia y Técnica, FONCYT (2013-1254) and Secretaría de Ciencia y Técnica, Universidad Nacional de Córdoba.
ML and WRA are members of the CONICET.
+ Corresponding author: mlaurito@conicet.gov.ar
https://orcid.org/0000-0002-6846-5029
Received 18 June 2019
Accepted 05 November 2019

Citation: Laurito M, Ontivero IM, Almirón WR. Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae). Mem Inst Oswaldo Cruz. 2019; 114: e190208.

Our Location

Memórias do Instituto Oswaldo Cruz

Av. Brasil 4365, Castelo Mourisco 
sala 201, Manguinhos, 21040-900 
Rio de Janeiro, RJ, Brazil

Tel.: +55-21-2562-1222

This email address is being protected from spambots. You need JavaScript enabled to view it.

Support Program

logo fiocruz logo governo
logo faperj logo cnpq marca capes