Malaria diagnosis from pooled blood samples: comparative analysis of real-time PCR, nested PCR and immunoassay as a platform for the molecular and serological diagnosis of malaria on a large-scale Giselle FMC Lima; José E Levi; Marcelo P Geraldi; Maria Carmen A Sanchez; Aluísio AC Segurado; Angélica D Hristov; Juliana Inoue; Maria de Jesus Costa-Nascimento; Silvia M Di Santi PAGE: 691-700 | DOI: 10.1590/S0074-02762011000600008

Malaria seroprevalence in blood bank donors from endemic and non-endemic areas of Venezuela Carmen Elena Contreras; Marcos de Donato; María Ana Rivas; Hectorina Rodulfo; Robert Mora; María Eulalia Batista; Norka Marcano PAGE: 123-129 | DOI: 10.1590/S0074-02762011000200002

Aseptic meningitis caused by Leptospira spp diagnosed by polymerase chain reaction Eliete Caló Romero; Roberta Morozetti Blanco; Paulo Hideki Yasuda PAGE: 988-992 | DOI: 10.1590/S0074-02762010000800007

Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats Nelson Mendes Marra; Fernanda Chiuso-Minicucci; Gabriel Capella Machado; Sofia Fernanda Gonçalves Zorzella-Pezavento; Thaís Graziela Donegá França; Larissa Lumi Watanabe Ishikawa; Alessandro FT Amarante; Alexandrina Sartori; Mônica RV Amarante PAGE: 57-61 | DOI: 10.1590/S0074-02762010000100008

Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre Aline Fagundes, ; Armando Schubach; Cíntia Cristiane de Paula; Alessandra Bogio; Liliane de Fátima Antonio; Patrícia Botelho Schiavoni; Vivian de Souza Monteiro; Maria de Fátima Madeira; Leonardo Pereira Quintella; Claudia Maria Valete-Rosalino; Erica de Camargo Ferreira e Vasconcellos; Rilza Beatriz Gayoso de Azeredo-Coutinho; Rachel S Pacheco; Mauro CA Marzochi; Keyla BF Marzochi PAGE: 109-112 | DOI: 10.1590/S0074-02762010000100018

Ocular toxoplasmosis - an update and review of the literature Alessandra G Commodaro; Rubens N Belfort; Luiz Vicente Rizzo; Cristina Muccioli; Claudio Silveira; Miguel N Burnier Jr; Rubens Belfort Jr PAGE: 345-350 | DOI: 10.1590/S0074-02762009000200030

Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control Belkisyolé Alarcón de Noya; Raiza RuizGuevara; Cecilia Colmenares; Sandra Losada; Oscar Noya PAGE: 29-35 | DOI: S0074-02762006000900006

Histopathological examination of nerve samples from pure neural leprosy patients: obtaining maximum information to improve diagnostic efficiency Sérgio Luiz Gomes Antunes; Leila Chimelli; Márcia Rodrigues Jardim; Robson Teixeira Vital; José Augusto da Costa Nery; Suzana Corte-Real; Mariana Andréa Vilas Boas Hacker; Euzenir Nunes Sarno PAGE: 246-253 | DOI: 10.1590/S0074-02762012000200015

Coccidioidomycosis in armadillo hunters from the state of Ceará, Brazil Raimunda Sâmia Nogueira Brillhante; Renato Evando Moreira Filho; Marcos Fábio Gadelha Rocha; Débora de Souza Collares Maia Castelo-Branco; Maria Auxiliadora Bezerra Fechine; Rita Amanda Chaves de Lima; Yuri Vieira Cunha Picanço; Rossana de Aguiar Cordeiro; Zoilo Pires de Camargo; José Ajax Nogueira Queiroz; Roberto Wagner Bezerra de Araujo; Jacó Ricarte Lima de Mesquita; José Júlio Costa Sidrim PAGE: 813-815 | DOI: 10.1590/S0074-02762012000600017

The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease Belkisyolé Alarcón de Noya; Zoraida Díaz-Bello; Cecilia Colmenares; Reinaldo Zavala-Jaspe; Teresa Abate; Rosa Contreras; Sandra Losada; Domingo Artigas; Luciano Mauriello, Raiza Ruiz-Guevara; Oscar Noya PAGE: 893-898 | DOI: 10.1590/S0074-02762012000700009

The combination of three faecal parasitological methods to improve the diagnosis of schistosomiasis mansoni in a low endemic setting in the state of Ceará, Brazil  Marta Cristhiany Cunha Pinheiro; Teiliane Rodrigues Carneiro; Ana Lúcia de Paula Hanemann; Sara Menezes de Oliveira; Fernando Schemelzer Moraes Bezerra PAGE: 873-876 | DOI: 10.1590/S0074-02762012000700006

Polymerase chain reaction for the evaluation of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil  Gabriel Costa de Carvalho; Letícia Helena dos Santos Marques; Luciana Inácia Gomes; Ana Rabello; Luiz Cláudio Ribeiro; Kezia Katiane Gorza Scopel; Sandra Helena Cerrato Tibiriçá; Elaine Soares Coimbra; Clarice Abramo PAGE: 899-902 | DOI: 10.1590/S0074-02762012000700010

Seroreactivity to new Mycobacterium leprae protein antigens in different leprosy-endemic regions in Brazil  Emerith Mayra Hungria; Regiane Morillas de Oliveira; Ana Lúcia Osório Maroclo de Souza; Maurício Barcelos Costa; Vânia Nieto Brito de Souza; Eliane Aparecida Silva; Fátima Regina Vilani Moreno; Maria Esther Salles Nogueira; Maria Renata Sales Nogueira Costa; Sônia Maria Usó Ruiz Silva; Samira Bührer-Sékula; Steven G Reed; Malcolm S Duthie; Mariane Martins de Araújo Stefani PAGE: 104-111 | DOI: 10.1590/S0074-02762012000900017

The potential for vaccination in leprosy elimination: new tools for targeted interventions Malcolm S Duthie; Paul Saunderson; Steven G Reed PAGE: 190-196 | DOI: 10.1590/S0074-02762012000900027

Genetic polymorphism in Taenia solium metacestodes from different Brazilian geographic areas  Ivanildes Solange da Costa Barcelos; Maria Aparecida Souza; Janethe Deolinda de Oliveira Pena; Gleyce Alves Machado; Lísia Gomes Martins de Moura; Julia Maria Costa-Cruz PAGE: 24-30 | DOI: 10.1590/S0074-02762012000100004

Use of the Kala-Azar Detect® and IT-LEISH® rapid tests for the diagnosis of visceral leishmaniasis in Brazil  Vanessa Peruhype-Magalhães; Tália Santana Machado-de-Assis; Ana Rabello PAGE: 951-952 | DOI: 10.1590/S0074-02762012000700019

Evaluation of two coproscopic techniques for the diagnosis of schistosomiasis in a low-transmission area in the state of Minas Gerais, Brazil Liliane Maria Vidal Siqueira; Paulo Marcos Zech Coelho; Áureo Almeida de Oliveira; Cristiano Lara Massara; Nídia Francisca de Figueiredo Carneiro; Anna Carolina Lustosa Lima; Martin Johannes Enk PAGE: 844-850 | DOI: 10.1590/S0074-02762011000700010

Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis Gardenia Braz Figueiredo Carvalho; Rosiane Aparecida da Silva-Pereira; Lucila Grossi Gonçalves Pacífico; Cristina Toscano Fonseca PAGE: 837-843 | DOI: 10.1590/S0074-02762011000700009

Use of a saline gradient for the diagnosis of schistosomiasis Paulo Marcos Z Coelho ; Arnon D Jurberg; Áureo A Oliveira; Naftale Katz PAGE: 720-723 | DOI: 10.1590/S0074-02762009000500010

Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae Abraham Rocha ; Cynthia Braga; Marcela Belém; Arturo Carrera; Ana Aguiar-Santos; Paula Oliveira; Maria José Texeira; André Furtado PAGE: 621-625 | DOI: 10.1590/S0074-02762009000400015

Congenital toxoplasmosis: evaluation of serological methods for the detection of anti-Toxoplasma gondii IgM and IgA antibodies IMX Rodrigues; AM Castro; MBF Gomes; WN Amaral; MM Avelino PAGE: 434-440 | DOI: 10.1590/S0074-02762009000300006

Diagnosis of congenital toxoplasmosis: prenatal and neonatal evaluation of methods used in Toulouse University Hospital and incidence of congenital toxoplasmosis MH Bessières ; A Berrebi; S Cassaing; J Fillaux; JP Cambus; A Berry; C Assouline ; JM Ayoubi; JF Magnaval PAGE: 389-392 | DOI: 10.1590/S0074-02762009000200038

Therapy, diagnosis and prognosis of chronic Chagas disease: insight gained in Argentina Sergio Sosa-Estani ; Rodolfo Viotti; Elsa Leonor Segura PAGE: 167-180 | DOI: 10.1590/S0074-02762009000900023

Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children Yvone Maia Brustoloni ; Rosimar Batista Lima; Rivaldo Venâncio da Cunha; Maria Elizabeth Dorval; Elisa Teruya Oshiro; Ana Lúcia Lyrio de Oliveira; Claude Pirmez PAGE: 497-500 | DOI: 10.1590/S0074-02762007005000036

Polymerase chain reaction with two molecular targets in mucosal leishmaniasis' diagnosis: a validation study Clemencia Ovalle Bracho ; Luisa Porras de Quintana; Sandra Muvdi Arenas; Melania Rios Parra PAGE: 549-554 | DOI: 10.1590/S0074-02762007005000061

A new miracidia hatching device for diagnosing schistosomiasis Arnon D Jurberg; Áureo A de Oliveira; Henrique L Lenzi; Paulo Marcos Z Coelho PAGE: 112-114 | DOI: S0074-02762008000100019

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens Ana Cláudia Carvalho Gouveia; Kathleen D. Eisenach; Solange Alves Vinhas; Fabiola Karla Corrêa Ribeiro; Renata Lyrio Peres; Reynaldo Dietze; David Jamil Hadad; Moisés Palaci PAGE: 386-391 | DOI: S0074-02762008000400012

Evaluation of adenosine deaminase seric activity in the diagnosis of bovine tuberculosis Márcio Roberto Silva; Pedro Moacyr Pinto Coelho Mota; Ricardo de Miranda Henriques Leite; Francisco Carlos Faria Lobato; Rômulo Cerqueira Leite; Andrey Pereira Lage PAGE: 391-395 | DOI: S0074-02762006000400008

Peripheral eosinophilia as an indicator of meningitic angiostrongyliasis in exposed individuals Kittisak Sawanyawisuth; Kanlayanee Sawanyawisuth; Vichai Senthong; Panita Limpawattana; Pewpan M Intapan; Somsak Tiamkao; Suthipun Jitpimolmard; Verajit Chotmongkol; Elizabeth Barrett-Connor PAGE: 942-944 | DOI: 10.1590/S0074-02762010000700020

Rural tourism: a risk factor for schistosomiasis transmission in Brazil Martin Johannes Enk; Graciela Larissa Amaral; Matheus Fernandes Costa e Silva; Denise Silveira-Lemos; Andréa Teixeira-Carvalho; Olindo Assis Martins-Filho; Rodrigo Correa-Oliveira; Giovanni Gazinnelli; Paulo Marcos Zech Coelho; Cristiano Lara Massara PAGE: 537-540 | DOI: 10.1590/S0074-02762010000400032

Access to diagnosis and treatment of Chagas disease/infection in endemic and non-endemic countries in the XXI century Luís Villa; Sílvia Morote; Oscar Bernal; Daniel Bulla; Pedro Albajar-Vinas PAGE: 87-94 | DOI: 10.1590/S0074-02762007005000081

Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis Zélia Maria Profeta Luz; Alexandre Rotondo da Silva; Fernanda de Oliveira Silva; Rachel B Caligiorne; Edward Oliveira; Ana Rabello PAGE: 62-66 | DOI: S0074-02762009000100010

Efficacy of benznidazol treatment for asymptomatic chagasic patients from state of Rio Grande do Sul evaluated during a three years follow-up CD Fernandes; FM Tiecher; MM Balbinot; DB Liarte; D Scholl; M Steindel; A Romanha PAGE: 27-32 | DOI: S0074-02762009000100004

Comparison of serological responses in two different populations with pulmonary tuberculosis Zaida Araujo; Francesca Giampietro; Lucio Castellano Cançado; Mahavir Singh; Albina Wide PAGE: 661-667 | DOI: S0074-02762008000700006

Serodiagnosis of human neurocysticercosis using antigenic components of Taenia solium metacestodes derived from the unbound fraction from jacalin affinity chromatography Gleyce Alves Machado, Heliana Batista de Oliveira, Margareth Leitão Gennari-Cardoso, José Roberto Mineo, Julia Maria Costa-Cruz PAGE: 368-375 | DOI: 10.1590/0074-0276108032013016

Parvovirus B19 infections in state of Rio de Janeiro, Brasil: 526 sera analyzed by IgM-enzyme-linked immunosorbent assay and polymerase chain reaction MCL Mendonça, SB Ribeiro, JNSS Couceiro, MG von Hubinger PAGE: 847-852

Detection of Lymnaea columella Infection by Fasciola hepatica through Multiplex-PCR Kelly Grace Magalhães, Liana Konovaloff Jannotti Passos, Omar dos Santos Carvalho PAGE: 421-424

Comparison between Splenic and Lymph Node Aspirations as Sampling Methods for the Parasitological Detection of Leishmania chagasi Infection in Dogs Stella Maria Barrouin-Melo, Daniela Farias Larangeira, Joelma Trigo, Paulo Henrique Palis Aguiar, Washington Luís Conrado dos-Santos, Lain Pontes-de-Carvalho PAGE: 195-197

Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction Elizabeth Córdoba Lanús, José Enrique Piñero, Ana Cristina González, Basilio Valladares,Mercedes Lizarralde de Grosso, Oscar Daniel Salomón PAGE: 187-192

Diagnosis of Brazilian vesiculoviruses by reverse transcription- polymerase chain reaction Daniela Wey Bonutti, Luiz Tadeu Moraes Figueiredo PAGE: 193-196

Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis Eliza Yoshie do Rosário, Odair Genaro, João C França-Silva, Roberto T da Costa, Wilson Mayrink, Alexandre Barbosa Reis*, Mariângela Carneiro PAGE: 197-203

Strongyloides venezuelensis Alkaline Extract for the Diagnosis of Human Strongyloidiasis by Enzyme-linked Immunosorbent Assay Eleuza Rodrigues Machado, Marlene Tiduko Ueta, Maria do Rosário de Fátima Gonçalves-Pires, João Batista Alves de Oliveira, Lúcia Helena Faccioli, Julia Maria Costa-Cruz PAGE: 849-851

Prevalence of Clostridium spp. and Clostridium difficile in Children with Acute Diarrhea in São Paulo City, Brazil Claudia EA Ferreira, Viviane Nakano, Edison L Durigon, Mario J Avila-Campos PAGE: 451-454

Use of Polymerase Chain Reaction and Enzymatic Cleavage in the Identification of Helicobacterspp. in Gastric Mucosa of Human Beings from North Paraná, Brazil PL Camargo, AA Alfieri, APFRL Bracarense, R Menoli, SR Spinosa, MK Hagiwara PAGE: 265-268

Clinical and Epidemiological Aspects of Human Parvovirus B19 Infection in an Urban Area in Brazil (Niterói City Area, State of Rio de Janeiro, Brazil) Solange Artimos de Oliveira,Luiz Antonio Bastos Camacho, Antonio Carlos de Medeiros Pereira, Tereza Filomena Faillace, Sérgio Setúbal,Jussara Pereira do Nascimento PAGE: 965-970

First Characterization of Candida albicans by Random Amplified Polymorphic DNA Method in Nicaragua and Comparison of the Diagnosis Methods for Vaginal Candidiasis in Nicaraguan Women Martha Darce Bello, Alcides Gonzalez, Christian Barnabé, Georges Larrouy PAGE: 985-989

Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis Henk DFH Schallig, Marilene Canto-Cavalheiro, Eduardo S da Silva PAGE: 1015-1018

Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy Adalberto Rezende Santos, Vivian Balassiano, Maria Leide W Oliveira, Marcia Aparecida da Silva Pereira, Patricia Barros Santos, Wim Maurits Degrave, Philip Noel Suffys PAGE: 1129-1133

Dengue in the State of Rio de Janeiro, Brazil, 1986-1998 Rita Maria R Nogueira,Marize P Miagostovich, Hermann G Schatzmayr, Flávia B dos Santos, Eliane S M de Araújo, AnaMaria B de Filippis, Rogério V de Souza, Sonia Maris O Zagne, Cecília Nicolai, Mary Baran, Gualberto Teixeira Filho PAGE: 297-304

Comparison between Human Immunodeficiency Virus Positive and Negative Patients with Tuberculosis in Southern Brazil Lucélia Henn, Fabiano Nagel, Felipe Dal Pizzol PAGE: 377-381

Improved Methods for the Diagnosis of African Trypanosomosis DE Rebeski, EM Winger, B Rogovic, MM Robinson, JR Crowther, RH Dwinger PAGE: 249-253

Circulating Antigens Levels in Different Clinical Forms of the Schistosoma mansoni Infection Yerkes Pereira e Silva, Evan Secor, Marcela Orsine Andrade, Naftale Katz, Ana Rabello PAGE: 83-86

Evaluation of DNA Recombinant Methodologies for the Diagnosis of Plasmodium falciparum and their Comparison with the Microscopy Assay L Urdaneta, P Guevara, JL Ramirez PAGE: 639-646

Diagnosis of Mucocutaneous Herpetic Infections by PCR without DNA Extraction Mauricio L Nogueira, Alex F Carvalho, Edel F Barbosa, Claudio A Bonjardim, Paulo CP Ferreira,Erna G Kroon PAGE: 213-214

Dried Blood Spots Collected on Filter Paper: An International Resource for the Diagnosis and Genetic Characterization of Human Immunodeficiency Virus Type-1 Sharon A Cassol, Stanley Read, Bruce G Weniger, Perry Gomez, Normand Lapointe, Chin-Yih Ou,P George Babu PAGE: 351-358

Diagnosis of Malaria by Acridine Orange Fluorescent Microscopy in an Endemic Area of Venezuela Irene Bosch, Carmen Bracho, Hilda A Pérez PAGE: 83-86

Recent Advances in the Diagnosis of Schistosoma Infection: the Detection of Parasite DNA Ana Rabello, Luís André Pontes, Emmanuel Dias-Neto PAGE: 171-172

Schistosomiasis Mansoni in Bananal (State of São Paulo, Brazil). I. Efficiency of Diagnostic and Treatment Procedures Horacio Manuel Santana Teles, Maria Esther de Carvalho, Cláudio Santos Ferreira, Fabiana Zacharias, Valquíria Rosa de Lima, Maria Lucia Condino Fadel PAGE: 181-186

Diagnostic challenges of single plaque-like lesion paucibacillary leprosy Raquel Rodrigues Barbieri, Anna Maria Sales, Ximena Illarramendi, Milton Ozório Moraes, José Augusto da Costa Nery, Suelen Justo Maria Moreira, Euzenir Nunes Sarno, Alice de Miranda Machado, Fernando Augusto Bozza PAGE: 944-947 | DOI: 10.1590/0074-0276140212

Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems Camila Ximenes, Eduardo Brandão, Paula Oliveira, Abraham Rocha, Tamisa Rego, Rafael Medeiros, Ana Aguiar-Santos, João Ferraz,Christian Reis, Paulo Araujo, Luiz Carvalho, Fabio L Melo PAGE: 978-983 | DOI: 10.1590/0074-0276140155

Evolution of Knowledge on the Etiological Diagnosis of Chagasic Infection Alejandro O Luquetti PAGE: 283-284

Changes in the Hemoculture Methodology Improve the Test Positivity Zélia M Profeta Luz PAGE: 295-298

Chagas Disease Diagnosis Using Polymerase Chain Reaction, Hemoculture and Serologic Methods Egler Chiari PAGE: 299-300

Diagnosis of Trypanosoma cruzi Chronic Infections in Humans: Usefulness of the Complement Regulatory Protein Antigens and Lytic Antibodies in the Control of Cure AU Krettli PAGE: 301-304

Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis Ciro Martins Gomes, Suleimy Cristina Mazin, Elisa Raphael dos Santos, Mariana Vicente Cesetti, Guilherme Albergaria Brízida Bächtold, João Henrique de Freitas Cordeiro, Fabrício Claudino Estrela Terra Theodoro, Fabiana dos Santos Damasco, Sebastián Andrés Vernal Carranza,Adriana de Oliveira Santos, Ana Maria Roselino, Raimunda Nonata Ribeiro Sampaio PAGE: 157-165 | DOI: 10.1590/0074-02760140280

Clinical and epidemiological profiles of individuals with drug-resistant tuberculosis Heloisa da Silveira Paro Pedro, Susilene Maria Tonelli Nardi, Maria Izabel Ferreira Pereira, Rosângela Siqueira Oliveira, Philip Noel Suffys, Harrison Magdinier Gomes, Amanda Juliane Finardi, Eloise Brasil de Moraes, Ida Maria Foschiani Dias Baptista, Ricardo Luiz Dantas Machado, Lilian Castiglioni PAGE: 235-241 | DOI: 10.1590/0074-02760140316

Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area Liliane Maria Vidal Siqueira, Luciana Inácia Gomes, Edward Oliveira, Eduardo Ribeiro de Oliveira, Áureo Almeida de Oliveira, Martin Johannes Enk, Nídia Figueiredo Carneiro, Ana Rabello, Paulo Marcos Zech Coelho PAGE: 209-214 | DOI: 10.1590/0074-02760140375

A Scarifier for Obtaining Specimens for Diagnosis of Leishmaniasis and Other Skin Infections Roberto Daibes Naiff PAGE: 87

Analysis of Respiratory Syncytial Virus in Clinical Samples by Reverse Transcriptase-Polymerase Chain Reaction Restriction Mapping Angel Valdivia, Clara Savón, Danay Chacón, Luis Sarmiento, Luis Morier, Anselmo Otero, Yudira Soto, Suset Oropesa, Angel Goyenechea PAGE: 389-393

Evaluation of Popular Stains for the Diagnosis of American Cutaneous Leishmaniasis Carmen Manuela Castillo, Carlos Rojas PAGE: 531-532

Comparison between Two Methods for Diagnosis of Trichinellosis: Trichinoscopy and Artificial Digestion María Laura Vignau, Mónica del Valle Guardis, Miguel Atilio Risso, Diego Fernando Eiras PAGE: 585-587

Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction Marize Pereira Miagostovich, Flávia Barreto dos Santos, Eliane Saraiva M de Araújo, Juarez Dias, Hermann G Schatzmayr, Rita Maria Ribeiro Nogueira PAGE: 595-600

Diagnosing Schistosomiasis Ana Rabello PAGE: 669-676

Multibacillary leprosy patients with high and persistent serum antibodies to leprosy IDRI diagnostic-1/LID-1: higher susceptibility to develop type 2 reactions Danielle de Freitas Mizoguti, Emerith Mayra Hungria, Aline Araújo Freitas, Regiane Morillas Oliveira, Ludimila Paula Vaz Cardoso, Mauricio Barcelos Costa, Ana Lúcia Maroclo Sousa, Malcolm S Duthie, Mariane Martins Araújo Stefani PAGE: 914-920 | DOI: 10.1590/0074-02760150198

Commercial enzyme-linked immunosorbent assay versus polymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis Pedro Emmanuel Alvarenga Americano do Brasil, Rodolfo Castro, Liane de Castro PAGE: 1-19 | DOI: 10.1590/0074-02760150296

The use of rapid dengue diagnostic tests in a routine clinical setting in a dengue-endemic area of Colombia Lyda Osorio, Marcela Uribe, Gloria Ines Ardila, Yaneth Orejuela, Margarita Velasco, Anilza Bonelo, Beatriz Parra PAGE: 510-516 | DOI: 10.1590/0074-02760140359

Scoring clinical signs can help diagnose canine visceral leishmaniasis in a highly endemic area in Brazil Kleverton Ribeiro da Silva, Vitor Rosa Ramos de Mendonça, Kellen Matuzzy Silva, Leopoldo Fabrício Marçal do Nascimento, Antonio Ferreira Mendes-Sousa, Flaviane Alves de Pinho, Manoel Barral-Netto, Aldina Maria Prado Barral, Maria do Socorro Pires e Cruz PAGE: 53-62 | DOI: 10.1590/0074-02760160305

Enzyme-linked immunosorbent assay for diagnosis of Amphimerus spp. liver fluke infection in Humans William Cevallos, Manuel Calvopiña, Victoria Nipáz, Belén Vicente-Santiago, Julio López-Albán, Pedro Fernández-Soto, Ángel Guevara, Antonio Muro PAGE: 364-369 | DOI: 10.1590/0074-02760160426

Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella Priscila T Baschirotto, Marco A Krieger, Leonardo Foti PAGE: 428-436 | DOI: 10.1590/0074-02760160509

The use of circulating cathodic antigen rapid test and serology for diagnosis of active Schistosoma mansoni infection in migrants in Italy, a non-endemic country: a cross sectional study Laura Infurnari, Laura Galli, Alba Bigoloni, Alessia Carbone, Stefania Chiappetta, Angelo Sala, Norberto Ceserani, Adriano Lazzarin, Antonella Castagna, Giovanni Gaiera PAGE: 452-455 | DOI: 10.1590/0074-02760160355

Chikungunya virus: clinical aspects and treatment Rivaldo V da Cunha, Karen S Trinta PAGE: 523-531 | DOI: 10.1590/0074-02760170044

Glutaraldehyde test for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis in an area with high tuberculosis incidence Ben Hadj Hassine Ahmed, Marzouk Manel, Dhaou Mohamed, Boukadida Jalel DOI: 10.1590/0074-02760160541

ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy Filipe R Lima, Iukary Takenami, Maurílio AL Cavalcanti, Lee W Riley, Sérgio Arruda DOI: 10.1590/0074-02760160549

Validation of the Dual-path Platform chromatographic immunoassay (DPP® CVL rapid test) for the serodiagnosis of canine visceral leishmaniasis Fabiano Borges Figueiredo, Tassia Cristina Bello de Vasconcelos, Maria de Fátima Madeira, Rodrigo Caldas Menezes, Ana Nilce Silveira Maia-Elkhoury, Andreza Pain Marcelino, Guilherme L Werneck DOI: 10.1590/0074-02760180260

Molecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vector Annhymariet Torrellas, Elizabeth Ferrer, Israel Cruz, Héctor de Lima, Olinda Delgado, José Carrero Rangel, José Arturo Bravo, Carmen Chicharro, Ivonne Pamela Llanes-Acevedo, Michael A Miles, María Dora Feliciangeli DOI: 10.1590/0074-02760180323

Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria Natália Ketrin Almeida-de-Oliveira, Otacílio C Moreira, Aline Rosa de Lavigne, Natália Ketrin Almeida-de-Oliveira, Otacílio C Moreira, Aline Rosa de Lavigne, Leila Mendonça-Lima, Guilherme Loureiro Werneck, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz DOI: 10.1590/0074-02760180350

Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis Anna Raquel Ribeiro dos Santos, Ângela Vieira Serufo, Maria Marta Figueiredo, Lara Carvalho Godoi, Jéssica Gardone Vitório, Andreza Pain Marcelino, Daniel Moreira de Avelar, Fernandes Tenório Gomes Rodrigues, George Luiz Lins Machado-Coelho, Fernanda Alvarenga Cardoso Medeiros, Selma Maria Bezerra Jerônimo, Edward José de Oliveira, Frederico Crepaldi Nascimento, Santuza Maria Ribeiro Teixeira, Ricardo Tostes Gazzinelli, Ronaldo Alves Pinto Nagem, Ana Paula Fernandes DOI: 10.1590/0074-02760180405

Diagnosis of Schistosoma mansoni infections: what are the choices in Brazilian low-endemic areas? Vanessa Silva-Moraes, Lisa M Shollenberger, Liliane Maria Vidal Siqueira, William Castro-Borges, Donald A Harn, Rafaella Fortini Queiroz e Grenfell, Ana Lucia Teles Rabello, Paulo Marcos Zech Coelho DOI: 10.1590/0074-02760180478

Where, when, and how the diagnosis of human visceral leishmaniasis is defined: answers from the Brazilian control program João Gabriel Guimarães Luz, Amanda Gabriela de Carvalho,Danilo Bueno Naves, João Victor Leite Dias, Cor Jesus Fernandes Fontes DOI: 10.1590/0074-02760190253

Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens Armando Menezes-Neto, Marcia da Costa Castilho, Guilherme Amaral Calvet, Edna Oliveira Kara, Camila Helena Aguiar Bôtto-Menezes, Marcus Vinícius Guimarães Lacerda, Gerson Fernando Mendes Pereira, Silvana Pereira Giozza, Ximena Pamela Diaz Bermudez, Noemia Santana Lima, Kayvon Modjarrad, Nathalie Broutet, Ana Maria Bispo de Filippis, Rafael Freitas Oliveira Franca on behalf of ZIKABRA Study Team* DOI: 10.1590/0074-02760200339

Dengue diagnostics: serious inaccuracies are likely to occur if pre-analytical conditions are not strictly followed Felipe Campos de Melo Iani, Ana Carolina Barbosa Caetano, Jéssica Caroline Wenceslau Cocovich, Frederico Figueiredo Amâncio, Maira Alves Pereira, Talita Émile Ribeiro Adelino, Sérgio Caldas, Marcos Vinícius Ferreira Silva, Glauco de Carvalho Pereira, Myrian Morato Duarte   DOI: 10.1590/0074-02760200287

Validation of ELISA with recombinant antigens in serological diagnosis of canine Leishmania infantum infection Mahyumi Fujimori, Arleana do Bom Parto Ferreira de Almeida, Stella Maria Barrouin-Melo, Luiz Ricardo Paes de Barros Cortez, Malcolm Scott Duthie, Roberto Mitsuyoshi Hiramoto, Flaviane Alves de Pinho, Steven Gregory Reed, Valéria Régia Franco Sousa, Nazaré Fonseca Souza, Rodrigo Martins Soares, José Eduardo Tolezano, Maria Carmen Arroyo Sanchez, Hiro Goto DOI: 10.1590/0074-02760200428

 COVID-19 diagnosis by RT-qPCR in alternative specimens Cássia Cristina Alves Gonçalves, Shana Priscila Coutinho Barroso, Alice Laschuk Herlinger, Rafael de Mello Galliez, Tailah Bernardo de Almeida, Lidia Theodoro Boullosa, Erica Ramos dos Santos Nascimento, Jessica M de Almeida, Raissa Mirella dos Santos Cunha da Costa, Tatiana Monteiro da Paixão, José Nelson dos Santos Silva Couceiro, Thiago Silva Frauches, Wilson Rodrigues de Souza Junior, Andréa Ribeiro Costa, Débora Souza Faffe, Isabela de Carvalho Leitão, Bianca Ortiz da Silva, Guilherme Sant’Anna de Lira, Isabela Labarba Carvalho de Almeida, Orlando da Costa Ferreira Junior, UFRJ-Covid19 Workgroup, Terezinha Marta Pereira Pinto Castiñeiras, Diana Mariani, Amilcar Tanuri DOI: 10.1590/0074-02760210085

Parasitological, serological and molecular diagnosis of acute and chronic Chagas disease: from field to laboratory Alejandro Gabriel Schijman, Julio Alonso-Padilla, Silvia Andrea Longhi, Albert Picado DOI: 10.1590/0074-02760200444

Epidemiology of paracoccidioidomycosis in Venezuela: a retrospective study from 1954 to 2019 Primavera Alvarado, Marcus de Melo Teixeira, Elsy Cavallera, Hugo Costa Paes, Giovanni Guerra, Gerardo Santander, Rommie Merino-Alado DOI: 10.1590/0074-02760210203

Accuracy of rapid point-of-care serological tests for leprosy diagnosis: a systematic review and meta-analysis Carmen Phang Romero, Rodolfo Castro, Pedro Emmanuel A do Brasil, Daniella R Pereira, Roberta Olmo Pinheiro, Cristiana M Toscano, Maria Regina Fernandes de Oliveira DOI: 10.1590/0074-02760220317

A review of serological tests available in Brazil for intestinal schistosomiasis diagnosis Lidia Mara da Silva Ramos, Rosiane A da Silva-Pereira, Edward Oliveira, Cristina Toscano Fonseca, Carlos Graeff-Teixeira DOI: 10.1590/0074-02760220236

Cerebrospinal fluid: a target of some fungi and an overview Danielly Corrêa-Moreira, Rodolfo Castro, Gisela Lara da Costa, Reginaldo Gonçalves Lima-Neto, Manoel Marques Evangelista Oliveira DOI: 10.1590/0074-02760220251

Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography Hevandro de Souza Campos, Edimilson Domingos da Silva, Gerson Silva de Lima, Rafael de Oliveira Resende, Patricia Burth DOI: 10.1590/0074-02760220265