Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction Anahí Souto Vieira; Grácia Maria Soares Rosinha; Carina Elisei de Oliveira; Silvio Arruda Vasconcellos; Paulo Andre Lima-Borges; Walfrido Moraes Tomás; Guilherme Miranda Mourão; Ana Cristina Reis Lacerda; Cleber Oliveira Soares; Flábio Ribeiro de Araújo; Ubiratan Piovezan; Carlos Andre Zucco; Aiesca Oliveira Pellegrin PAGE: 763-768 | DOI: 10.1590/S0074-02762011000600019

Aseptic meningitis caused by Leptospira spp diagnosed by polymerase chain reaction Eliete Caló Romero; Roberta Morozetti Blanco; Paulo Hideki Yasuda PAGE: 988-992 | DOI: 10.1590/S0074-02762010000800007

High prevalence and low E6 genetic variability of human papillomavirus 58 in women with cervical cancer and precursor lesions in Southeast Mexico Jaqueline Canul Canche; Iván Rosado López; Nicolás G Suárez; Gladis Colli Acosta; Laura Conde-Ferráez; Thelma Canto de Cetina; María R González Losa PAGE: 144-148 | DOI: 10.1590/S0074-02762010000200006

Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats Nelson Mendes Marra; Fernanda Chiuso-Minicucci; Gabriel Capella Machado; Sofia Fernanda Gonçalves Zorzella-Pezavento; Thaís Graziela Donegá França; Larissa Lumi Watanabe Ishikawa; Alessandro FT Amarante; Alexandrina Sartori; Mônica RV Amarante PAGE: 57-61 | DOI: 10.1590/S0074-02762010000100008

Comparison of HPV genotyping by type-specific PCR and sequencing Nara de Oliveira Carvalho; Dora Méndez del Castillo; Carlos Perone; José Nélio Januário; Victor Hugo de Melo; Geraldo Brasileiro Filho PAGE: 73-78 | DOI: 10.1590/S0074-02762010000100011

Polymerase chain reaction for the evaluation of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil  Gabriel Costa de Carvalho; Letícia Helena dos Santos Marques; Luciana Inácia Gomes; Ana Rabello; Luiz Cláudio Ribeiro; Kezia Katiane Gorza Scopel; Sandra Helena Cerrato Tibiriçá; Elaine Soares Coimbra; Clarice Abramo PAGE: 899-902 | DOI: 10.1590/S0074-02762012000700010

Unveiling healthy carriers and subclinical infections among household contacts of leprosy patients who play potential roles in the disease chain of transmission Sérgio Araújo; Janaína Lobato; Érica de Melo Reis; Dulcinéa Oliveira Bernardes Souza; Maria Aparecida Gonçalves; Adeilson Vieira Costa; Luiz Ricardo Goulart ; Isabela Maria Bernardes Goulart PAGE: 55-59 | DOI: 10.1590/S0074-02762012000900010

Prevalence of human papillomavirus and Epstein-Barr virus DNA in penile cancer cases from Brazil Larissa Alves Afonso; Natalia Moyses; Gilda Alves; Antônio Augusto Ornellas; Mauro Romero Leal Passos; Ledy do Horto dos Santos Oliveira; Silvia Maria Baeta Cavalcanti PAGE: 18-23 | DOI: 10.1590/S0074-02762012000100003

Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues  Aleco D'Andrea; Yarelys Zamora Martinez; Rosa Alduina; Vincenzo Monteverde; Carmen Fernandez Molina; Maria Vitale, PAGE: 85-88 | DOI: 10.1590/S0074-02762012000100012

Diagnosis of congenital toxoplasmosis: prenatal and neonatal evaluation of methods used in Toulouse University Hospital and incidence of congenital toxoplasmosis MH Bessières ; A Berrebi; S Cassaing; J Fillaux; JP Cambus; A Berry; C Assouline ; JM Ayoubi; JF Magnaval PAGE: 389-392 | DOI: 10.1590/S0074-02762009000200038

First encounter of subclinical human Leishmania (Viannia) infection in State of Rio Grande do Sul, Brazil Aline Fagundes; Mauro CA Marzochi; Octavio Fernandes; Mauricio A Perez; Armando O Schubach; Tânia MP Schubach; Maria RR Amendoeira; Eliame Mouta-Confort; Keyla BF Marzochi PAGE: 1003-1005 | DOI: 10.1590/S0074-02762007000800018

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens Ana Cláudia Carvalho Gouveia; Kathleen D. Eisenach; Solange Alves Vinhas; Fabiola Karla Corrêa Ribeiro; Renata Lyrio Peres; Reynaldo Dietze; David Jamil Hadad; Moisés Palaci PAGE: 386-391 | DOI: S0074-02762008000400012

The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases Mari Tuyama; Renata F Boente; Maria C Rebelo; Ricardo P Igreja; David E Barroso PAGE: 138-142 | DOI: S0074-02762008000200003

Evaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia Demetrio L Valle Jr; Jeannie I Andrade; Esperanza C Cabrera; Windell L Rivera PAGE: 117-122 | DOI: 10.1590/S0074-02762010000200001

Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children Thiago Leite Fraga; Yvone Maia Brustoloni; Rosimar Baptista Lima; Maria Elizabeth Cavalheiros Dorval; Elisa Teruya Oshiro; Janaina Oliveira; Ana Lúcia Lyrio de Oliveira; Claude Pirmez PAGE: 310-313 | DOI: S0074-02762010000300011

Alternative PCR protocol using a single primer set for assessing DNA quality in several tissues from a large variety of mammalian species living in areas endemic for leishmaniasis Eduardo C Ferreira; Célia M Gontijo; Israel Cruz; Maria Norma Melo; Aristóbolo M Silva PAGE: 895-898 | DOI: 10.1590/S0074-02762010000700009

Molecular approaches for eco-epidemiological studies of Paracoccidioides brasiliensis Virgínia Bodelão Richini-Pereira; Sandra de Moraes Gimenes Bosco; Raquel Cordeiro Theodoro; Severino Assis da Graça Macoris; Eduardo Bagagli PAGE: 636-643 | DOI: 10.1590/S0074-02762009000400018

Asymptomatic Plasmodium spp. infection in Tierralta, Colombia Zulma Milena Cucunubá; Ángela Patricia Guerra; Sonia Judith Rahirant; Jorge Alonso Rivera; Liliana Jazmín Cortés; Rubén Santiago Nicholls PAGE: 668-673 | DOI: S0074-02762008000700007

Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices C Rigotto; CK Kolesnikovas; V Moresco; CMO Simões; CRM Barardi PAGE: 970-974 | DOI: 10.1590/S0074-02762009000700005

Risk factors associated with human papillomavirus infection in two populations from Rio de Janeiro, Brazil Katia C Silva; Maria Luiza Garcia Rosa; Natalia Moyses; Larissa A Afonso; Ledy HS Oliveira; Silvia MB Cavalcanti PAGE: 885-891 | DOI: 10.1590/S0074-02762009000600011

Detection of human herpesvirus 7 infection in young children presenting with exanthema subitum Ivna de Melo Magalhães; Rebeca Vazquez Novo Martins; Renata Oliveira Vianna; Natalia Moysés; Larissa Alves Afonso; Solange Artimos Oliveira; Silvia Maria Baeta Cavalcanti PAGE: 371-373 | DOI: 10.1590/S0074-02762011000300020

A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia Stephanie Wagner Gallo, Patrícia Locosque Ramos, Carlos Alexandre Sanchez Ferreira, Sílvia Dias de Oliveira PAGE: 390-391 | DOI: 10.1590/0074-0276108032013020

Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients Praphathip Eamsobhana; Darawan Wanachiwanawin; Naowarat Dechkum; Anchana Parsartvit; Hoi Sen Yong PAGE: 116-118 | DOI: 10.1590/S0074-02762013000100020

The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region Lina Marcela Villa, Felipe Guhl, Daniel Zabala, Juan David Ramírez, Daniel Alfonso Urrea, Diana Carolina Hernández, Zulma Cucunubá, Marleny Montilla, Julio César Carranza, Karina Rueda, Jorge Eduardo Trujillo, Gustavo Adolfo Vallejo PAGE: 932-935 | DOI: 10.1590/0074-0276130201

Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? Daniela Camargos Costa, Ana Paula Madureira, Lara Cotta Amaral, Bruno Antônio Marinho Sanchez, Luciano Teixeira Gomes, Cor Jésus Fernandes Fontes, Jean Ezequiel Limongi, Cristiana Ferreira Alves de Brito, Luzia Helena Carvalho PAGE: 21-28 | DOI: 10.1590/0074-0276140102

A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area Teiliane Rodrigues Carneiro, Regina Helena Saramago Peralta, Marta Cristhiany Cunha Pinheiro, Sara Menezes de Oliveira, José Mauro Peralta, Fernando Schemelzer Moraes Bezerra PAGE: 1037-1044 | DOI: 10.1590/0074-0276130202

Detection of Mycobacterium leprae DNA by Polymerase Chain Reaction in the Blood of Individuals, Eight Years after Completion of Anti-leprosy Therapy Adalberto Rezende Santos, Vivian Balassiano, Maria Leide W Oliveira, Marcia Aparecida da Silva Pereira, Patricia Barros Santos, Wim Maurits Degrave, Philip Noel Suffys PAGE: 1129-1133

Inhibition of the Polymerase Chain Reaction by Sputum Samples from Tuberculosis Patients After Processing Using a Silica-guanidiniumthiocyanate DNA Isolation Procedure Philip Suffys, Patricia Rosa Vanderborght, Patricia Barros dos Santos, Leticia Almeida Pinto Correa, Yolanda Bravin, Afranio Lineu Kritski PAGE: 1137-1139

Parasite Persistence in Treated Chagasic Patients Revealed by Xenodiagnosis and Polymerase Chain Reaction Constança Britto, Celeste Silveira, Maria Angelica Cardoso, Patricia Marques, Alejandro Luquetti, Vanize Macêdo, Octavio Fernandes PAGE: 823-826

Comparison of Polymerase Chain Reaction on Fresh Tissue Samples and Fecal Drops on Filter Paper for Detection of Trypanosoma cruzi in Rhodnius prolixus PL Dorn, J Flores, B Brahney, A Gutierrez, R Rosales*, A Rodas*, C Monroy* PAGE: 503-505

Genetic Markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) Obtained by the Double Stringency Polymerase Chain Reaction Technique Roseli Tuan, Paula Cristina Bortolato PAGE: 435-436

Diagnosis of Mucocutaneous Herpetic Infections by PCR without DNA Extraction Mauricio L Nogueira, Alex F Carvalho, Edel F Barbosa, Claudio A Bonjardim, Paulo CP Ferreira,Erna G Kroon PAGE: 213-214

The Schistosome Genome Project: RNA Arbitrarily Primed- PCR Allows the Accelerated Generation of Expressed Sequence Tags Emmanuel Dias Neto, Richard Harrop, Rodrigo Corrêa-Oliveira, Sérgio DJ Pena, R Alan Wilson, Andrew JG Simpson PAGE: 655-657

Stained Smears as a Source of DNA Jackeline Alger, Martha C Acosta, Caterin Lozano, Carmelita Velasquez, Luz Angela Labrada PAGE: 589-591

Natural Infection of a Domestic Cat (Felis domesticus) with Leishmania (Viannia) in the Metropolitan Region of Belo Horizonte, State of Minas Gerais, Brazil Valéria MA Passos, Edson B Lasmar, Célia MF Gontijo, Octavio Fernandes, Wim Degrave PAGE: 19-20

Phlebotomine fauna, natural infection rate and feeding habits of Lutzomyia cruzi in Jaciara, state of Mato Grosso, Brazil Veruska Nogueira de Brito, Arleana do Bom Parto Ferreira de Almeida, Luciano Nakazato, Rosemere Duarte, Cladson de Oliveira Souza, Valéria Régia Franco Sousa PAGE: 899-904 | DOI: 10.1590/0074-0276140112

Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients Karla Lopes Mandu de Campos, Ana Paula Machado, Flávia Gatto de Almeida, Camila Mareti Bonin, Thiago Theodoro Martins Prata, Larissa Zatorre Almeida, Cacilda Tezelli Junqueira Padovani, Alda Maria Teixeira Ferreira, Carlos Eurico dos Santos Fernandes, Inês Aparecida Tozetti PAGE: 352-355 | DOI: 10.1590/0074-0276130397

Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems Camila Ximenes, Eduardo Brandão, Paula Oliveira, Abraham Rocha, Tamisa Rego, Rafael Medeiros, Ana Aguiar-Santos, João Ferraz,Christian Reis, Paulo Araujo, Luiz Carvalho, Fabio L Melo PAGE: 978-983 | DOI: 10.1590/0074-0276140155

Quantification of C4d deposition and hepatitis C virus RNA in tissue in cases of graft rejection and hepatitis C recurrence after liver transplantation Alice Tung Wan Song, Evandro Sobroza de Mello, Venâncio Avancini Ferreira Alves, Norma de Paula Cavalheiro, Carlos Eduardo Melo, Patricia Rodrigues Bonazzi, Fatima Mitiko Tengan, Maristela Pinheiro Freire, Antonio Alci Barone, Luiz Augusto Carneiro D'Albuquerque, Edson Abdala PAGE: 56-64 | DOI: 10.1590/0074-02760140192

Comparison of Some Molecular-genetic Techniques for Identification of Leishmania Circulating in Natural Foci of Zoonotic Cutaneous Leishmaniasis in the Central Asia Region Margarita V Strelkova, Raquel S Pacheco, Sergey A Bulat,Tatjana A Rakitskaya PAGE: 109-114

Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina Regina Helena Saramago Peralta, Jorge Néstor Velásquez, Flavia de Souza Cunha, María Laura Pantano, Fernando Campos Sodré, Sidnei da Silva, Osvaldo Germán Astudillo, José Mauro Peralta, Silvana Carnevale PAGE: 30-36 | DOI: 10.1590/0074-02760150303

Comparison of LAMP and PCR for molecular mass screening of sand flies for Leishmania martiniquensis infection Saruda Tiwananthagorn, Hirotomo Kato, Ranchana Yeewa, Amontip Muengpan, Raxsina Polseela, Saovanee Leelayoova PAGE: 100-107 | DOI: 10.1590/0074-02760160254

Comparison of four molecular approaches to identify Candida parapsilosis complex species Leonardo Silva Barbedo, Maria Helena Galdino Figueiredo-Carvalho, Mauro de Medeiros Muniz, Rosely Maria Zancopé-Oliveira PAGE: 214-219 | DOI: 10.1590/0074-02760160412

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil Sandra Yamashiro, Vagner Ricardo da Silva Fiuza, Ângela Therezinha Lauand Sampaio Teixeira, Nilson Branco, Carlos Emílio Levy, Isabel Cristina Vidal Siqueira de Castro, Regina Maura Bueno Franco PAGE: 404-410 | DOI: 10.1590/0074-02760160435

Simple Protocol for population (Sanger) sequencing for Zika virus genomic regions Gabriela Bastos Cabral, João Leandro de Paula Ferreira, Renato Pereira de Souza, Mariana Sequetin Cunha, Adriana Luchs, Cristina Adelaide Figueiredo, Luís Fernando de Macedo Brígido PAGE: 38-44 | DOI: 10.1590/0074-02760170248

Multiplex PCR assays developed for neglected pathogen detection in undifferentiated acute febrile illness cases in tropical regions Leidi Carvajal Aristizabal, Karl Ciuoderis, Laura S Pérez-Restrepo, Jorge E Osorio, Juan P Hernández-Ortiz DOI: 10.1590/0074-02760240053